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You are here:Home » Antibodies » Antibodies-Albumin » Anti -Ovalbumin, Denatured

Anti -Ovalbumin, Denatured


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Ovalbumin comprises about 60% of the solids in chicken egg white. Native ovalbumin is a globular monomer of molecular mass 45kD (1). It is structurally related to both serpins and lipcalins.
Catalog #O8075-34
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:2500; Strong reaction is seen with Ovalbumin directly coated onto the microtiter well
Western Blot: 1:2000; Reacts with Ovalbumin in reduced and unreduced forms after SDS-PAGE.
Optimal dilutions to be determined by the researcher.
Culture MediumRPMI 1640 with 10% fetal calf serum.
HybridomaX63-Ag8.653 myeloma cells with spleen cells from Balb/c
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No6F161
FormSupplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
PurityPurified by Protein A/G affinity chromatography from culture supernatant.
ImmunogenOvalbumin isolated from chicken egg white, denatured by heat and formaldehyde.
SpecificityRecognizes denatured chicken ovalbumin; but cross-reacts with native OVA. Cross-reactivity to egg albumins from other species has not been tested. Epitope specificity differs from that of clones 6F162 and 6F163, as determined by inhibition ELISA.
Reacts with OA in denatured or modified forms (native OVA surface-adsorbed, heat-denatured or reduced). Strong reaction is seen in ELISA, with OA directly coated onto the microtiter well (3). In Western Blot, recognizes both denatured and native OVA adsorbed onto nitrocellulose; also recognizes both reduced and unreduced OVA forms.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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