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P3113-50 Mouse Anti-Parvalbumin

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Frog
Swiss Prot
P20472, P02625, P32848, P84535, Q0VCG3, P02627
Isotype
IgG1
Clone Number
2Q137 (PARV-19)
Grade
Ascites
Applications
IC IHC WB
Crossreactivity
Bo Ca Fe Fr Gt Hu Mo Po Rb Rt
Accession #
NM_002854.2
Shipping Temp
Blue Ice
Storage Temp
-20°C

Parvalbumin is a calcium binding albumin protein. It has three EF hand motifs and is structurally related to calmodulin and troponin C. Parvalbumin is localized in fast-contracting muscles, where its levels are highest, and in the brain and some endocrine tissues. Parvalbumin is present in GABAergic interneurons in the nervous system, predominantly expressed by chandelier and basket cells in the cortex.

Applications
Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 using 10ug of mouse brain lysate. Immunocytochemistry: Cultured neurons Immunohistochemistry: 1:1000-1:2000 (Indirect Immunoperoxidase). Works on formalin fixed, paraffin embedded tissue sections of rat cerebellum. Fixation does not appear to affect ability to detect parvalbumin. Optimal dilution determined by the researcher.
Recommended Secondary Antibodies
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-14C: IgG, (HRP) Pab Gt x Mo I1904-19C: IgG, (HRP) Pab Gt x Mo
Recommended Control
Brain tissue, cultured neurons
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Parvalbumin purified from frog muscle.
Form
Supplied as a liquid in 0.09% sodium azide.
Purity
Ascites
Specificity
Recognizes frog Parvalbumin at ~12kD. Reacts with parvalbumin from brain and muscle. Directed against an epitope at the first Ca+2-binding site and specifically stains the Ca+2-bound form of parvalbumin. Species Crossreactivity: human, rat, bovine, porcine, feline, rabbit, canine, fish, mouse and goat
References
1. Perrotti, L.I., et al., J. Neurosci. 24(47): 10,594-10,602 (2004). 2. Wu, M., et al., J. Neurosci. 22: 7754-7765 (2002). 3. Bender, R.A., et al., European Journal of Neuroscience 13: 679-686 (2001).
USBio References
No references available
Conjugates
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