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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -PCNA (Proliferating Cell Nuclear Antigen) (Biotin)

Anti -PCNA (Proliferating Cell Nuclear Antigen) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IH IC IF
PCNA, originally known as Cyclin, is a 36kD nuclear protein associated with the cell cycle. It has recently been identified as the polymerase delta accessory protein. PCNA is essential for cellular DNA synthesis. It is also required for the in vitro replication of simian virus 40 (SV 40) DNA, where it acts to coordinate reading and lagging strand synthesis at the replication fork. This antibody is a useful tool for studying the proliferating cells in normal tissues, and possible aberrant expression in neoplasm. It is useful for staining the nuclei in proliferating calls during the G1, S, G2, and M stages. It has been suggested that high expression of PCNA correlates with organ metastasis and vascular invasion, and may be an effective prognostic factor (2).
Catalog #P3115-08C
ApplicationsSuitable for use in Immunofluorescence, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:500-1:2000
Immunohistochemistry: 1:5-1:20 (Alcohol or formalin-fixed, paraffin-embedded).
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0-Ag14 myeloma cells with spleen cells from BALB/c mice.
Recommended Positive ControlsHeLa cells, A431 cells, WI-38 human fibroblasts, Xenopus cytosol and NIH 3T3 cells.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3G236
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide, 50% glycerol. Labeled with Biotin.
PurityPurified
ImmunogenRecombinant PCNA polypetides.
SpecificityRecognizes human PCNA. Reacts with all vertebrate species, insects and S. pombe on Western Blots.
Species CrossreactivityMouse, rat, chicken, Xenopus.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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