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You are here:Home » Antibodies » Antibodies-Carbohydrates, Glycoproteins » Anti -Peptidoglycan, Bacterial

Anti -Peptidoglycan, Bacterial


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Clone Host Grade Applications
Monoclonal Mouse Ascites E IC
Peptidoglycan is a polymer of glycopeptide linking N-acetyl or N-glycorylmuramic acid and peptide containing D-amino acid, and is a constituent of the cell wall in most prokaryotes.  It is known that this substance is pyrogenic and initiates a variety of physiological activities such as pyrogenicity and reactivity with SLP Reagent 1 body fluid from silkworm.
Catalog #P3290-05A
ApplicationsSuitable for use in ELISA, Dot Blot and Immunocytochemistry. Reported by an independent laboratory to be suitable for use in Immunohistochemistry (frozen & paraffin) and Western Blot. Other applications have not been tested.
Recommended DilutionsELISA: Competitive or solid phase.
Optimal dilutions to be determined by the researcher.
Procedural NoteAlthough PG is a bacterial surface component, pretreatments, such as treatment with strong acid, for Gram-positive bacteria, or with a detergent, such as SDS, for Gram-negative bacteria, may be necessary to expose the epitopes.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No2Q1862
ConcentrationNot determined
FormSupplied as a liquid. No preservatives added.
ImmunogenInsoluble PG obtained by TCA-heat and ethanol extraction of Streptococcus mutans BHT cells.
SpecificityRecognizes the three-dimensional polymer complex structure of PG. The epitopes appear to consist of discontinuous glycan and/or amino acid residues and have not been fully defined. In a competitive immunoassay format, several compounds were found to be ineffective as inhibitors: muramyldipeptide, N-acetylglucosamine (NAG), chitin (polymer of NAG) and acid-hydrolyzed chitin (NAG oligomers).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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