Suitable for use in ELISA, Dot Blot and Immunocytochemistry. Reported by an independent laboratory to be suitable for use in Immunohistochemistry (frozen & paraffin) and Western Blot. Other applications have not been tested.

ELISA: Competitive or solid phase. Optimal dilutions to be determined by the researcher.

Although PG is a bacterial surface component, pretreatments, such as treatment with strong acid, for Gram-positive bacteria, or with a detergent, such as SDS, for Gram-negative bacteria, may be necessary to expose the epitopes.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Insoluble PG obtained by TCA-heat and ethanol extraction of Streptococcus mutans BHT cells.

Supplied as a liquid. No preservatives added.

Ascites.

Recognizes the three-dimensional polymer complex structure of PG. The epitopes appear to consist of discontinuous glycan and/or amino acid residues and have not been fully defined. In a competitive immunoassay format, several compounds were found to be ineffective as inhibitors: muramyldipeptide, N-acetylglucosamine (NAG), chitin (polymer of NAG) and acid-hydrolyzed chitin (NAG oligomers).