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Anti -Peptidoglycan, Streptococcus mutans (PG)
Anti -Peptidoglycan, Streptococcus mutans (PG)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Ascites |
E IC |
|
| Peptidoglycan is a polymer of glycopeptide linking N-acetyl or N-glycorylmuramic acid and peptide containing D-amino acid, and is a constituent of the cell wall in most prokaryotes. It is known that this substance is pyrogenic and initiates a variety of physiological activities such as pyrogenicity and reactivity with SLP Reagent 1 body fluid from silkworm. | | | Catalog # | P3290-05A | | Applications | Suitable for use in ELISA, Dot Blot and Immunocytochemistry. Reported by an independent laboratory to be suitable for use in Immunohistochemistry (frozen & paraffin) and Western Blot. Other applications have not been tested. | | Recommended Dilutions | ELISA: Competitive or solid phase. | | Immunocytochemistry: Although PG is a bacterial surface component, pretreatments, such as treatment with strong acid, for Gram-positive bacteria, or with a detergent, such as SDS, for Gram-negative bacteria, may be necessary to expose the epitopes. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 2Q1862 | | Host | Mouse | | Concentration | Not Determined | | Form | Supplied as a liquid. No preservatives added. | | Purity | Ascites | | Immunogen | Insoluble PG obtained by TCA-heat and ethanol extraction of Streptococcus mutans BHT cells. | | Specificity | Recognizes the three-dimensional polymer complex structure of PG. The epitopes appear to consist of discontinuous glycan and/or amino acid residues and have not been fully defined. In a competitive immunoassay format, several compounds were found to be ineffective as inhibitors: muramyldipeptide, N-acetylglucosamine (NAG), chitin (polymer of NAG) and acid-hydrolyzed chitin (NAG oligomers). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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