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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E B

Phosphorylation of tyrosine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation
Catalog #	P4078-25A
Hybridoma	Fusion between mouse splenocytes and mouse myeloma Sp2/0
Applications	Suitable for use in ELISA and Immunoblot. Other applications not tested.
Recommended Dilution	ELISA: 1:2000-1:10,000 Use HRP at anti mouse IgG for detection
	Immunoblot: 1:1000; anti-phosphotyrosine is used at room temperature for 60 min followed by detection using IRDye™800 Conjugated Goat-anti-Mouse IgG (H&L) diluted 1:2500 for 30' at room temperature.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	5H21
Host	Mouse
Concentration	Not determined
Form	Supplied as a liquid, 0.02% sodium azide. No stabilizers or preservatives added. No stabilizing proteins added.
Purity	Ascites
Immunogen	Phosphotyrosine conjugated to KLH
Specificity	Reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates. Minimal crossreactivity against phosphoserine or phosphothreonine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.