USBio Logo

P4078-25A Mouse Anti-Phosphotyrosine

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Isotype
IgG1,k
Clone Number
13F9
Grade
Ascites
Applications
E IC IF WB
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free

Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (5). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (6).

Applications
Suitable for use in ELISA, Immunocytochemistry, Immunofluorescence and Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1:100 Immunofluorescence (IC): 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Phosphotyrosine conjugated to KLH
Form
Supplied as a liquid, 0.02% sodium azide. No stabilizers or preservatives added. No stabilizing proteins added.
Purity
Ascites
Specificity
Recognizes free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates. Minimal crossreactivity against phosphoserine or phosphothreonine.
References
1. Sarmay, G., et al., (1994) Proc. Natl. Acad. Sci. USA 91, 4140. 2. Arad-Dann, H., (1993) J. Histochem. Cytochem. 41, 513. 3. Dvir, A., et al., (1991) J. Cell Biol. 113, 857. 4. Far, D.F., et al., (1994) Cytometry 15, 327. 5. Barry, S.T., and Critchley, D.R. (1994) J. Cell Sci. 107, 2033.
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved