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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Phosphotyrosine

Anti -Phosphotyrosine

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B
Phosphorylation of tyrosine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation
Catalog #P4078-25A
HybridomaFusion between mouse splenocytes and mouse myeloma Sp2/0
ApplicationsSuitable for use in ELISA and Immunoblot. Other applications not tested.
Recommended DilutionELISA: 1:2000-1:10,000 Use HRP at anti mouse IgG for detection
Immunoblot: 1:1000; anti-phosphotyrosine is used at room temperature for 60 min followed by detection using IRDye™800 Conjugated Goat-anti-Mouse IgG (H&L) diluted 1:2500 for 30' at room temperature.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No5H21
HostMouse
ConcentrationNot determined
FormSupplied as a liquid, 0.02% sodium azide. No stabilizers or preservatives added. No stabilizing proteins added.
PurityAscites
ImmunogenPhosphotyrosine conjugated to KLH
SpecificityReacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates. Minimal crossreactivity against phosphoserine or phosphothreonine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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