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You are here:Home » Antibodies » Abs to Oncoproteins » Anti -Pim1 (Proviral Integration Site 1, PIM-1)

Anti -Pim1 (Proviral Integration Site 1, PIM-1)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IH IC
Protein kinase Pim-1 is a serine/threonine kinase that has been implicated in the development of hematopoietic and prostatic malignancies. Two isoforms, the 44 and 33kDa Pim-1, are expressed in all human prostate cancer cell lines examined. The subcellular localization of human 44kDa Pim-1 is primarily on the plasma membrane, while the 33kDa isoform is present in both the cytosol and nucleus in PCA cells. The nuclear location of Pim-1 is essential for its regulation of the levels of HDM2 protein, and possibly for additional biological activities of this protein kinase. Studies imply a physiological role of the Pim-1 protooncogene during hematopoietic development and a deregulation in various leukemias. Pim-1 is capable of enhancing the rate of occurrence of c-Myc-induced lymphomas, and functions to block factor-withdrawal and genotoxic stress-induced apoptosis. During human fetal hematopoiesis Pim-1 is highly expressed in the liver and spleen. In contrast, at the adult stage it is only slightly expressed in circulating granulocytes.
Catalog #P4186-60D
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000-1:10,000
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E446
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues in human Pim1. Species Sequence Homology: mouse and rat
SpecificityRecognizes human Pim1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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