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Anti -Pim1 (Proviral Integration Site 1, PIM-1)
Anti -Pim1 (Proviral Integration Site 1, PIM-1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IH IC |
|
| Protein kinase Pim-1 is a serine/threonine kinase that has been implicated in the development of hematopoietic and prostatic malignancies. Two isoforms, the 44 and 33kDa Pim-1, are expressed in all human prostate cancer cell lines examined. The subcellular localization of human 44kDa Pim-1 is primarily on the plasma membrane, while the 33kDa isoform is present in both the cytosol and nucleus in PCA cells. The nuclear location of Pim-1 is essential for its regulation of the levels of HDM2 protein, and possibly for additional biological activities of this protein kinase. Studies imply a physiological role of the Pim-1 protooncogene during hematopoietic development and a deregulation in various leukemias. Pim-1 is capable of enhancing the rate of occurrence of c-Myc-induced lymphomas, and functions to block factor-withdrawal and genotoxic stress-induced apoptosis. During human fetal hematopoiesis Pim-1 is highly expressed in the liver and spleen. In contrast, at the adult stage it is only slightly expressed in circulating granulocytes. | | | Catalog # | P4186-60D | | Applications | Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. | | Recommended Dilutions | Western Blot: 1:1000-1:10,000 | | Immunohistochemistry: 1:100-1:250 | | Immunocytochemistry: 1:100-1:250 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 9E446 | | Host | Rabbit | | Source | Human | | Concentration | Not Determined | | Form | Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol. | | Purity | Supernatant | | Immunogen | A synthetic peptide corresponding to residues in human Pim1. Species Sequence Homology: mouse and rat | | Specificity | Recognizes human Pim1. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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