Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Golgi Proteins » Anti -PIST (PDZ Protein Interacting Specifically with TC10, CFTR-associated ligand, CAL, dJ94G16.2, Fused in Glioblastoma, FIG, Golgi-associated PDZ and Coiled-coil Motif Containing, Golgi Associated PDZ and Coiled Coil Motif Containing Protein, GOPC, GOPC1, OT

Anti -PIST (PDZ Protein Interacting Specifically with TC10, CFTR-associated ligand, CAL,
dJ94G16.2, Fused in Glioblastoma, FIG, Golgi-associated PDZ and Coiled-coil Motif
Containing, Golgi Associated PDZ and Coiled Coil Motif Containing Protein, GOPC, GOPC1, OT

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). PIST, a PDZ-containing protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1. PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner. PIST is a PDZ domain-containing Golgi protein.
Catalog #P4207-51B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:100,000
Western Blot: 0.05-0.2ug/ml. ~55kD band observed in human brain (frontal cortex) lysates (calculated MW of 50.5kD.
Optimal dilutions to be determined by the researcher.
Gene Symbol GOPC
Entrez:v57120 (Human)
Swiss Prot:vNP_065132. (Human)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in TBS, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide LDDLHTLYHKKSY, representing the C-Terminus of the human protein (residues 450-462) according to NP_065132.
SpecificityRecognizes human PIST (PDZ Protein Interacting Specifically with TC10). Species Crossreactivity: bovine, canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links