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Anti -PIWIL1 (Piwi-like Protein 1, HIWI)
Anti -PIWIL1 (Piwi-like Protein 1, HIWI)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IC |
|
| HIWI, also called PIWIL1 is an 861aa, 99kD (predicted) cytoplasmic protein of the PIWI subgroup of the Argonaute family. PIWI domains bind a class of ~29-30 nucleotide RNAs termed piRNAs. PIWIL1 orthologs are expressed with piRNAs in germ cells and are essential for spermatogenesis in mice. CD34+ hematopoietic progenitor cell PIWIL1 is downregulated upon differentiation. When overexpressed in leukemic cell lines, PIWIL1 inhibits growth and induces apoptosis. Human PIWIL1 shares 95% aa sequence identity with mouse and rat PIWIL1. | | | Catalog # | P4210-35D | | Applications | Suitable for use in Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Immunocytochemistry: 8-25ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 12K163 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml after reconstitution | | Form | Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml. | | Purity | Purified by Protein A/G affinity chromatography. | | Immunogen | Recombinant corresponding to aa82-290 from human PIWIL1/HIWI expressed in E.coli. | | Specificity | Recognizes human PIWIL1. No reactivity with recombinant human PIWIL2 or rhPIWIL4. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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