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You are here:Home » Antibodies » Abs to DNA Replication, Repair » Anti -PIWIL1 (Piwi-like Protein 1, HIWI)

Anti -PIWIL1 (Piwi-like Protein 1, HIWI)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IC
HIWI, also called PIWIL1 is an 861aa, 99kD (predicted) cytoplasmic protein of the PIWI subgroup of the Argonaute family. PIWI domains bind a class of ~29-30 nucleotide RNAs termed piRNAs. PIWIL1 orthologs are expressed with piRNAs in germ cells and are essential for spermatogenesis in mice. CD34+ hematopoietic progenitor cell PIWIL1 is downregulated upon differentiation. When overexpressed in leukemic cell lines, PIWIL1 inhibits growth and induces apoptosis. Human PIWIL1 shares 95% aa sequence identity with mouse and rat PIWIL1.
Catalog #P4210-35D
ApplicationsSuitable for use in Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunocytochemistry: 8-25ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No12K163
HostMouse
SourceHuman
Concentration~0.5mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenRecombinant corresponding to aa82-290 from human PIWIL1/HIWI expressed in E.coli.
SpecificityRecognizes human PIWIL1. No reactivity with recombinant human PIWIL2 or rhPIWIL4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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