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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -PPARg (Peroxisome Proliferator Activated Receptor, gamma)

Anti -PPARg (Peroxisome Proliferator Activated Receptor, gamma)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IP
Peroxisome proliferator-activated receptor, gamma (PPARgamma) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator (1). PPARg is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage (2). Besides its role in mediating adipogenesis and lipid metabolism (2), PPARg also modulates insulin sensitivity, cell proliferation and inflammation (3). PPAR gamma transcriptional activity is inhibited by MAP kinase phosphorylation of PPAR gamma at Ser84 (4,5).
Catalog #P5505-06B
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
PuritySerum
ImmunogenSynthetic peptide corresponding to the sequence around Asp69 of human PPAR gamma.
SpecificityRecognizes endogenous levels of total human PPAR gamma protein at 53kD (PPARg1) and 57kD (PParg2). Species crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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