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Anti -PRAS40 (Proline-Rich AKT Substrate)
Anti -PRAS40 (Proline-Rich AKT Substrate)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP |
|
| Proline-Rich AKT Substrate (PRAS40) is a 40kD substrate of AKT. Activated AKT phosphorylates PRAS40 on threonine 246, enabling PRAS40 to bind to 14-3-3. AKT and PRAS40 are components of the phosphoinositide 3-kinase (PI3K) pathway. This pathway plays a role in glucose uptake, cell growth and apoptosis inhibition. The precise function of PRAS40 is not yet known; however, it has been hypothesized that PRAS40 interacts with SH3 and WW domain containing proteins and may change the function of these proteins. | | | Catalog # | P5640 | | Applications | Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. | | Recommended Dilutions | : | | Western Blot: 0.1-1ug/ml | | Optimal dilutions to be determined by the researcher. | | Recommended Positive Controls | HeLa cells; Jurkat cells. | | Hybridoma | BALB/c mouse splenocytes were fused with FO myeloma cells. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 5J32 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide. | | Purity | Purified by Protein A/G affinity chromatography. | | Immunogen | Recombinant full length human PRAS40 expressed in E. coli. | | Specificity | Recognizes human PRAS40 at ~40kD. This band is absent from PRAS40 siRNA-treated HeLa cell extracts. Does not crossreact with mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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