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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -PRAS40 (Proline-Rich AKT Substrate)

Anti -PRAS40 (Proline-Rich AKT Substrate)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
Proline-Rich AKT Substrate (PRAS40) is a 40kD substrate of AKT. Activated AKT phosphorylates PRAS40 on threonine 246, enabling PRAS40 to bind to 14-3-3. AKT and PRAS40 are components of the phosphoinositide 3-kinase (PI3K) pathway. This pathway plays a role in glucose uptake, cell growth and apoptosis inhibition. The precise function of PRAS40 is not yet known; however, it has been hypothesized that PRAS40 interacts with SH3 and WW domain containing proteins and may change the function of these proteins.
Catalog #P5640
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions:
Western Blot: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlsHeLa cells; Jurkat cells.
HybridomaBALB/c mouse splenocytes were fused with FO myeloma cells.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No5J32
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenRecombinant full length human PRAS40 expressed in E. coli.
SpecificityRecognizes human PRAS40 at ~40kD. This band is absent from PRAS40 siRNA-treated HeLa cell extracts. Does not crossreact with mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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