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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -PRDM14 (PR Domain Zinc Finger Protein 14, PR Domain-containing Protein 14)

Anti -PRDM14 (PR Domain Zinc Finger Protein 14, PR Domain-containing Protein 14)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IH
PRDM14 (PR [PRDI-BF1 and RIZ] domain containing protein 14) is a 64kD (predicted) member of the PR Domain family of proteins. It is expressed in embryonic stem cells and multiple tumor cell types. PRDM14 likely demonstrates histone methyltransferase activity, serving as a transcriptional repressor. It is suggested to block cell differentiation and maintain cell self-renewal, but also be necessary for germ cell lineage commitment. Human PRDM14 is 571aa in length. It contains one SET domain (aa253-371) followed by six C2H2 type Zn finger motifs (aa400-568). Over aa1-201, human PRDM14 shares only 46% aa identity with mouse PRDM14.
Catalog #P5750-58
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 0.5-1ug/ml
Western Blot: 0.1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified, E. coli-derived, recombinant human PRDM14 (aa2-201; Accession #Q9GZV8).
SpecificityRecognizes human PRDM14.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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