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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum IH IF

Neuropeptide Y is a peptide belonging to the PP-family and occurs in neurons and adrenal medullary cells.
Catalog #	P6260
Applications	Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Immunofluorescence: 1:400-1:800 with overnight incubation at 4°C.
	Immunohistochemistry: frozen sections
	Optimal dilutions to be determined by the researcher.
Positive Control	Stefanini-fixed sections of rat small intestine.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	Not determined
Form	Supplied as a lyophilized powder. Reconstitute with 50-100ul of sterile ddH2O. Dilute further in 0.1M PBS, 1% BSA and 0.1% sodium azide.
Purity	Serum
Immunogen	Synthetic Prepro-NPY 68-97 (C-PON). Raised using a synthetic peptide from the prepro-NPY sequence which has no homologies to PP and NPY.
Specificity	Recognizes Preproneuropeptide Y. Antisera raised against NPY often crossreact with PP and PYY. Absorption with 10-100ug immunogen per ml diluted antiserum abolishes the staining. Species Crossreactivity: human and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.