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You are here:Home » Antibodies » Abs to Enzymes, Methyltransferase » Anti -PRMT6 (Protein arginine N Methyltransferase 6)

Anti -PRMT6 (Protein arginine N Methyltransferase 6)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. PRMT6 was identified by searching the human genome for protein arginine N-methyltransferase
(PRMT) family members. PRMT6 cDNA codes for a protein of approximate molecular weight of 42kD consisting of a catalytic core sequence common to other PRMT enzymes. PRMT6 demonstrates type I PRMT activity, capable of forming both >NG-monomethylarginine and asymmetric >NG,NG- dimethylarginine derivatives on the recombinant glycine- and arginine-rich substrates. A comparison of substrate specificity reveals that PRMT6 is functionally distinct from two previously characterized type I enzymes, PRMT1 and PRMT4. In addition to methylating substrate proteins, PRMT6 displays automethylation activit. GFP-fusion protein expression in the transfected cell lines suggest that PRMT6 localizes in the nucleus.
Catalog #P9004-30
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1,000
Optimal dilutions to be determined by the researcher.
positive control HeLa cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined.
FormSupplied as a liquid in PBS, 0.02% sodium azide, 0.1 mg/ml BSA.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptides corresponding to human PRMT6 amino acid sequence.
SpecificityRecognizes Human PRMT6 and mouse cell line (RAW).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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