Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:18000 Western Blot: 0.1-0.3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-NRFQIATVTE, aa230-239 of human PSMB4.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human PSMB4. Species sequence homology: Canine, mouse, rabbit, porcine and bovine.
References
1. Yoshida T, et al. International journal of molecular medicine 2010 Apr 25 (4): 649-56.