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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » Protein Phosphatase 2C alpha, Recombinant, Human (PP2Ca, PPM1A, Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A)

Protein Phosphatase 2C alpha, Recombinant, Human (PP2Ca, PPM1A, Protein Phosphatase,
Mg2+/Mn2+ Dependent, 1A)

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Specifications

Protein phosphatase 2C alpha, also called PP2Calpha, Protein Phosphatase, Magnesium-dependent type 1A, and PPM1A, dephosphorylates proteins with some preference for phospho-threonine over phospho-serine residues 1. It is insensitive to the PP1 and PP2a inhibitors such as okadaic acid 2, but has an absolute requirement for either magnesium or manganese ions 3 and can be activated by unsaturated fatty acids such as arachidonic and oleic acids 4 . Overexpression of PP2Calpha causes G2/M cell cycle arrest and apoptosis that has been associated with the activation of p53 5 . Dephosphorylation of proteins in the nucleus by PP2Calpha is suspected to play a role in terminating or reducing the sensitivity of the responses to SMADS 6 and stress-activated proteins such as p38 and JNK 7.
Catalog #P9107-37B
Source E. coli-expressed recombinant protein.
Molecular Mass Amino acid residues 1-324 of PP2Calpha (Accession # NP_808820) with a C-terminal His6 sequence has a predicted molecular weight of 36.8kD.
Activity Measured by the ability to dephosphorylate Serine/Threonine Phosphatase Substrate II which has the amino acid sequence KRT(PO3)IRR. Assayed in a buffer containing 50 mM Tris, 10 mM MgCl2, 1 mg/mL BSA, 0.02% Brij-35, pH 7.5 for 30 minutes at 37° C with shaking. 10 ng of enzyme cleaves 200 M of phosphopeptide substrate with a specific activity of > 2 moles/min/mg.
Storage and Stability This product is shipped on dry ice. Upon receiving, aliquot immediately and store at -70°C in a manual defrost freezer. Samples are stable for up to twelve months from the date of receipt. Avoid repeated freeze-thaw cycles.
SourceE. coli
Purity95%, as determined by SDS-PAGE and visualized by silver stain.
ConcentrationAs reported
FormSupplied as a liquid in 20mM HEPES, 50mM NaCl, 1mM MnCl2, 1mM DTT, pH 7.5.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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