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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E

Protein S is a vitamin K-dependent, potent neutral anticoagulant plasma glycoprotein. Protein S has a molecular weight of approximately 70kD and consists of a single polypeptide chain. The concentration of protein S in human plasma is 25ug/ml, with a half-life of approximately two days. About 40% of protein S in plasma is in a free form, wheraes 60% is complexed with C4b-binding Protein (C4BP). Only the free protein S functions as a cofactor to antigen presenting cells (APC) in the APC-dependent degradation of Factor Va and Factor VIIIa.
Catalog #	P9108-06C
Applications	Suitable for use in ELISA. Not suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions	Optimal dilutions to be determined by the researcher.
Hybridoma	X63-Ag8.653: Female CF1 Balb/c mice immunized by intraperitoneal injection.
Culture Medium	RPMI 1640 with 10% fetal calf serum.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Monoclonal
Isotype	IgG2b,k
Clone No	6F185
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Purity	Purified by Protein A/G affinity chromatography.
Immunogen	Protein S isolated from human plasma.
Specificity	Recognizes human Protein S. Reacts strongly with Protein S. Strong reaction is seen in sandwich ELISA in combination with a biotin-labeled antibody against protein S. Reactivity is Ca2+ dependent and is optimal when using Tris buffer containing 1mM calcium chloride. In Dot Blot, SDS-treatment abolishes the reactivity.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.