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Anti -PTEN Tumor Suppressor Protein (MMAC1, Phosphatase and Tensin, TEP1)
Anti -PTEN Tumor Suppressor Protein (MMAC1, Phosphatase and Tensin, TEP1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Supernatant |
IH |
|
| PTEN is a candidate tumor suppressor gene mapping to the homozygous deletion on human chromosome 10q23. It appears to be mutated at considerable frequency in human cancers. The predicted PTEN protein has a protein tyrosine phosphatase domain and extensive homology to tensin. Studies have shown that purified PTEN catalyzes dephosphorylation of PtdIns(3,4,5)P3, specifically at position 3 on the inositol ring, and raise the possibility that PTEN acts in vivo as a phosphoinositide 3-phosphatase by regulating PtdIns(3,4,5)P3 levels. PTEN has been implicated in a large number of human tumors and is conserved from humans to worms. Characterization of PTEN protein showed that it is a phosphatase that acts on proteins and on 3-phosphorylated phosphoinositides, and can therefore modulate signal-transduction pathways that involve lipid second messengers. Recent results indicate that at least part of its role is to regulate the activity of the serine/threonine kinase AKT/PKB, and thus influence cell survival signaling. | | | Although PTEN has the consensus sequence of a protein tyrosine phosphatase, it dephosphorylates p-nitrophenylphosphate and other synthetic and protein substrates poorly. The greatest catalytic activity has been observed with the highly negatively charged, multiply phosphorylated polymer of (Glu-Tyr)n. | | | Catalog # | P9182-03 | | Applications | Suitable for use in Immunohistochemistry (Formalin/paraffin). Other applications not tested. | | Recommended Dilution | Immunohistochemistry: 1:25–1:50 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. | | Optimal dilutions to be determined by the researcher. | | Cellular Localization | Cytoplasmic | | Positive Control | Prostate carcinoma. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Monoclonal | | Isotype | IgM,k | | Clone No | 0.N.540B | | Host | Mouse | | Source | Human | | Concentration | Not determined | | Form | Supplied as concentrated tissue culture supernatant., 0.1% sodium azide. | | Purity | Tissue culture supernatant | | Immunogen | Recombinant human PTEN protein | | Specificity | Recognizes a 60kD protein, which is identified as human PTEN/MMAC1, a tumor suppressor. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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