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You are here:Home » Antibodies » Abs to Tumor Suppressors » Anti -PTEN Tumor Suppressor Protein (MMAC1, Phosphatase and Tensin, TEP1)

Anti -PTEN Tumor Suppressor Protein (MMAC1, Phosphatase and Tensin, TEP1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant IH
PTEN is a candidate tumor suppressor gene mapping to the homozygous deletion on human chromosome 10q23. It appears to be mutated at considerable frequency in human cancers. The predicted PTEN protein has a protein tyrosine phosphatase domain and extensive homology to tensin. Studies have shown that purified PTEN catalyzes dephosphorylation of PtdIns(3,4,5)P3, specifically at position 3 on the inositol ring, and raise the possibility that PTEN acts in vivo as a phosphoinositide 3-phosphatase by regulating PtdIns(3,4,5)P3 levels. PTEN has been implicated in a large number of human tumors and is conserved from humans to worms. Characterization of PTEN protein showed that it is a phosphatase that acts on proteins and on 3-phosphorylated phosphoinositides, and can therefore modulate signal-transduction pathways that involve lipid second messengers. Recent results indicate that at least part of its role is to regulate the activity of the serine/threonine kinase AKT/PKB, and thus influence cell survival signaling.
Although PTEN has the consensus sequence of a protein tyrosine phosphatase, it dephosphorylates p-nitrophenylphosphate and other synthetic and protein substrates poorly. The greatest catalytic activity has been observed with the highly negatively charged, multiply phosphorylated polymer of (Glu-Tyr)n.
Catalog #P9182-03
ApplicationsSuitable for use in Immunohistochemistry (Formalin/paraffin). Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:251:50 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Cellular Localization Cytoplasmic
Positive Control Prostate carcinoma.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgM,k
Clone No0.N.540B
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as concentrated tissue culture supernatant., 0.1% sodium azide.
PurityTissue culture supernatant
ImmunogenRecombinant human PTEN protein
SpecificityRecognizes a 60kD protein, which is identified as human PTEN/MMAC1, a tumor suppressor.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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