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P9182-05H Rabbit Anti-PTEN Tumor Suppressor Protein (MMAC1, Phosphatase and Tensin, TEP1)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

PTEN (phosphatase and tensin homology on chromosome 10) dephosphorylates proteins and lipids such as AKT and phosphatidylinositol 3,4,5-triphosphate (PIP3) and therefore functions as a key regulatory enzyme in a central signal transduction pathway. It also interacts with proteins Shc and FAK to promote apoptosis when necessary and to regulate cell division and migration.1 PTEN is considered a tumor suppressor as loss-of-function mutations in PTEN often result in human cancers including melanoma and prostate carcinoma.2,3 PTEN is expressed in almost all tissues of the body as a 403 amino acid protein with an estimated molecular weight of 47kD.

Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 2ug/ml Immunohistochemistry (Formalin-fixed paraffin-embedded sections): 2ug/ml Optimal dilutions to be determined by the researcher.
Positive Controls
A431 and HEK293
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to aa254-270 (KVEFFHKQNKMLKKDKM) of human PTEN. Species sequence homology: human, chimpanzee, mouse, rat and canine.
Form
Supplied as a liquid in TBS, pH 7.4, 0.1% BSA, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human PTEN. Species Crossreactivity: mouse and rat.
References
1. Di Cristofano, A., and Pandolfi, P.P. The multiple roles of PTEN in tumor suppression. Cell 100, 387-390 (2000).|2. Vivanco, I., and Sawyers, C.L. The phosphatidylinositol 3-kinase-AKT pathway in human cancer. Nature Reviews.Cancer 2, 489-501 (2002).|3. Dahia, P.L.M. PTEN, a unique tumor suppressor gene. Endocrine-Related Cancer 7, 115-129 (2000).|4. Steck, P.A.,Pershouse, M.A.,Jasser, S.A., et al. Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers. Nature Genetics 15, 356-362 (1997).
USBio References
No references available
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