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You are here:Home » Antibodies » Abs to Tyrosine Kinases » Anti -Pyk2 (CAKbeta, cdk-activating Protein Kinase beta)

Anti -Pyk2 (CAKbeta, cdk-activating Protein Kinase beta)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IP IC
Catalog #P9400-09
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, IP-kinase assay and Immunocytochemistry.
Recommended DilutionImmunoblot Analysis: This lot of antibody at a 1:2000 dilution detected Pyk2 (RAFTK/CAK-b) in bovine brain cytosolic and PC12 cell RIPA lysates.
Immunoprecipitation: 2.5ul of this lot of antibody immunoprecipitated Pyk2 (RAFTK/CAK-b) from 250ug of PC12 RIPA lysate.
Immunoprecipitation Kinase Assay: Reported by an independent laboratory to immunoprecipitate Pyk2 (RAFTK/CAK-b) from CMK cell lysates. The immune complex subsequently phosphorylated a poly(Glu/Tyr) substrate peptide in vitro.
Immunocytochemistry: Reported by an independent laboratory to detect Pyk2 (RAFTK/CAK-b) in PC12 cells.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid containing 0.05% sodium azide before the addition of glycerol to 30%.
PuritySerum
ImmunogenGST-fusion protein containing aa681-1009 of human Pyk2
SpecificityRecognizes Pyk2 (RAFTK/CAK-b), Mr ~116kD. Nonspecific proteins at 98 and 60kD were also detected. Antibody designation
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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