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P9400-25 Rabbit Anti-Pyk2, phosphorylated (Tyr402) (CAKbeta, cdk-activating Protein Kinase beta)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Accession #
U33284
Shipping Temp
Blue Ice
Storage Temp
-20°C

Proline-rich/Ca2+-activated tyrosine kinase (Pyk2) is a member of the FAK family of non-receptor, proline-rich protein tyrosine kinases. Pyk2 signaling is initiated by a variety of extracellular stimuli including integrin ligation, CD surface marker ligation (e.g., CD3, CD28, TCR, VCAM), bioactive peptides, growth factors, cytokines, chemokines and certain stress stimuli (reactive oxygen species and Ca++ flux). Pyk2 is involved in the regulation of vesicular transport, osteoclastic bone resorption, modulation of ion channels, T- and B-cell receptor signaling and cell death. Pyk2 tyrosine 402 is autophosphorylated, an important initial step for full activation of the kinase. This phosphorylation also allows binding of Src and the p85 subunit of PI3-kinase.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Positive Control
Chick Embryo Fibroblast (CEF) cells expressing human Pyk2
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phosphopeptide corresponding to human Pyk2 that contains tyrosine 402. Species sequence homology: rat.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Pyk2 when phosphorylated at Tyr402. Species sequence homology: Rat and chicken Pyk2 (100%).
References
1. Fernandis, A.Z., et al. (2003) Differential regulation of CXCR4-mediated T-cell chemotaxis and mitogen-activated protein kinase activation by the membrane tyrosine phosphatase, CD45. J. Biol. Chem. 278(11):9536-9543 2. Frank, G.D., et al. (2003) Distinct mechanisms of receptor and nonreceptor tyrosine kinase activation by reactive oxygen species in vascular smooth muscle cells: role of metalloprotease and protein kinase C-delta. Mol. Cell. Biol 23(5):1581-1589 3. Shah, B.H., et al. (2003) Dependence of gonadotropin-releasing hormone-induced neuronal MAPK signaling on epidermal growth factor receptor transactivation. J. Biol. Chem. 278(5):2866-2875 4. Chen, H.C., et al. (2002) Activation of the ERK pathway and atypical protein kinase C isoforms in exercise- and aminoimidazole-4-carboxamide-1-beta-D-riboside (AICAR)-stimulated glucose transport. J. Biol. Chem. 277(26):23554- 23562 5. Cheng, J.J., et al. (2002) Cyclic strain activates redox-sensitive proline-rich tyrosine kinase 2 (PYK2) in endothelial cells. J. Biol. Chem. 277(50):48152-48157 6. Dunty, J.M. and M.D. Schaller (2002) The N termini of focal adhesion kinase family members regulate substrate phosphorylation, localization, and cell morphology. J. Biol. Chem. 277(47):45644-45654 7. Matsumoto, Y., et al. (2002) Possible involvement of the vascular endothelial growth factor-Flt-1-focal adhesion kinase pathway in chemotaxis and the cell proliferation of osteoclast precursor cells in arthritic joints. J. Immunol. 168(11):5824-5831 8. McCole, D.F., et al. (2002) Transactivation of the epidermal growth factor receptor in colonic epithelial cells by carbacholrequires extracellular release of transforming growth factor-alpha. J. Biol. Chem. 277(45):42603-42612 9. Melendez, J., et al. (2002) Activation of Pyk2/Related Focal Adhesion Tyrosine Kinase and Focal Adhesion Kinase in cardiac remodeling. J. Biol. Chem. 277(47):45203-45210 10. Sajan, M.P., et al. (2002) Sorbitol activates atypical protein kinase C and GLUT4 glucose transporter translocation/glucose transport through proline-rich tyrosine kinase-2, the extracellular signal-regulated kinase pathway and phospholipase D. Biochem. J. 362(Pt 3):665-674 11. Cary, L.A. and J.L. Guan (1999) Focal Adhesion Kinase in integrin-mediated signaling. Front. Biosci. 4:D102-D113.
USBio References
No references available
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