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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -Rab5 (Member RAS Oncogene Family, Ras-related Protein Rab-5, Rab GTPase)

Anti -Rab5 (Member RAS Oncogene Family, Ras-related Protein Rab-5, Rab GTPase)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Rab5 is a 24kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras
superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hypervariable COOH-terminal domains with a cysteine
motif implicated in subcellular targeting. Post-translational modification of the cysteine motif with
one or two geranyl groups is essential for the membrane association and correct intracellular
localization of Rab proteins(3). Each Rab shows a characteristic subcellular distribution (4). In particular, Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane. It regulates the fusion between endocytic vesicles and early endosomes, as well as the homotypic fusion between early endosomes (5). Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and EEA1 (6). Anti-Rab5 may be used as an early endosome marker.
Catalog #R0008-01D
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot:1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 1x PBS, 50% glycerol and 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman Rab5 synthetic peptide conjugated to KLH; identical to dog Rab5 sequence over the residues.
SpecificityRecognizes human Rab5. Species Crossreactivity: mouse, monkey, bovine, rat. Reactivity
to other species has not been tested.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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