RANTES (regulated upon activation, normal T-cell expressed and secreted) is a 70-amino acid CC chemokine originally cloned from T cell-specific sequences-enriched cDNA libraries. Both human and murine RANTES are chemotactic for monocytes. Human RANTES is also a chemoattractant for T cells and eosinophils, as well as an activator of basophils for histamine release and of eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. In contrast to other CC chemokines, RANTES is only slowly expressed after activation of leukocytes. It is involved in a number of pathological conditions like chronic inflammation and autoimmune diseases. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency (SIV).
Applications
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 0.5-1ug/ml. The detection limit under reducing conditions is 0.25-2.0ng/lane and under non-reducing conditions, 0.25-0.5ng/lane. ELISA (Sandwich): 2-4ug/ml in 100ul/well will detect at least 500pg/ml of recombinant human RANTES when used with R1100-03 at the detection antibody at 0.5-1ug/ml. Immunohistochemistry (FFPE): 10ug/ml. Requires HIER with citrate buffer, pH 6.0 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to human RANTES, expressed in E. coli.
Form
Supplied as a lyophilized powder from PBS. Reconstitute with 500ul sterile dH2O.
Purity
Purified by Protein A affinity chromatography from ascites
Specificity
Recognizes human RANTES.