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R1120-35 Mouse Anti-Rap2B (Member of RAS oncogene family, Ras-related protein Rap-2b precursor)

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P61225
Isotype
IgG2a
Clone Number
9H175
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Rap2B is a member of the small GTPase superfamily and ras family of proteins. Unlike normal ras proteins that have a nontransforming glutamine residue at aa 61, Rap2B has a threonine residue in that position. This change makes the intrinsic GTPase activity for Rap2B lower, thereby allowing it to exist in the activated state for a longer period of time than normal ras proteins. Aggregation of platelets by thrombin results in the activation of Rap2B in a Ca 2+ and PI3 kinase-dependent manner.

Applications
Suitable for Western Blot. Other applications not tested.
Storage
Lyophilized samples are stable for twelve months from date of receipt when stored at -20°C to -70°C. Upon reconstitution, the antibody can be stored at 2°-8°C for 1 month without detectable loss of activity. Reconstituted antibody can also be aliquotted and stored frozen at -20°C to -70°C in a manual defrost freezer for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Immunogen
E. coli-derived rhRap2B.
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography.
Specificity
This antibody detects endogenous human Rap2B at 22 kDa using Western blot. The |antibody does not cross-react with recombinant human Rap2A.
Conjugates
Pricing
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