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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Red Fluorescent Protein (RFP)

Anti -Red Fluorescent Protein (RFP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Well-characterized antibodies against short-sequence epitope tags are common in the study of protein expression systems.
RFP-tags are composed of 15 amino acid residue peptides derived from aa22-36 of red fluorescent protein (RFP) from the Discosoma sea anemone.
Catalog #R1310-02
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 0.05-0.2ug/ml
Western Blot: 0.1-1.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitue with 80ul sterile ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenPeptide corresponding to aa22-36, VNGHEFEIEGEGEGR from the Discosoma sea anemone, conjugated to KLH
SpecificityRecognizes fusion proteins containing RFP-tags and RFP variants, such as mCherry, tdTomato, DsRed, DsRed2, mOrange. Does not react with GFP or GFP-tagged proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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