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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

Well-characterized antibodies against short-sequence epitope tags are common in the study of protein expression systems.
RFP-tags are composed of 15 amino acid residue peptides derived from aa22-36 of red fluorescent protein (RFP) from the Discosoma sea anemone.
Catalog #	R1310-02
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	ELISA: 0.05-0.2ug/ml
	Western Blot: 0.1-1.0ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~0.5mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitue with 80ul sterile ddH2O.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Peptide corresponding to aa22-36, VNGHEFEIEGEGEGR from the Discosoma sea anemone, conjugated to KLH
Specificity	Recognizes fusion proteins containing RFP-tags and RFP variants, such as mCherry, tdTomato, DsRed, DsRed2, mOrange. Does not react with GFP or GFP-tagged proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.