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You are here:Home » Antibodies » Abs to Enzymes, Polymerase » Anti -RMP (Unconventional Prefoldin RPB5 Interactor, RNA Polymerase II Subunit 5-mediating Protein, Protein NNX3, RPB5-mediating Protein, C19orf2, NNX3, URI)

Anti -RMP (Unconventional Prefoldin RPB5 Interactor, RNA Polymerase II Subunit 5-mediating
Protein, Protein NNX3, RPB5-mediating Protein, C19orf2, NNX3, URI)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
RMP (RPB5-Mediating Protein), also known as URI (Unconventional prefoldin RBP5 Interactor), was recently described as an unconventional member of the prefoldin (PFD) family of chaperones that are involved in actin and tubulin folding (1-4). Like conventional members of the a-class of PFDs, RMP contains N- and C-terminal a-helical coiled-coil structures connected by two B hairpins. In addition, RMP possesses a RPB5-binding segment and a long C-terminal acidic segment. It is posited that RMP exists as a component of a macromolecular complex within human cells and functions as a molecular scaffold to assemble a PFD complex containing other PFDs and proteins with functions in transcription and ubiquitination. Indeed, evidence is provided that RMP negatively modulates RNA polymerase II-dependent transcription by binding to TFIIF (5) and RBP5 (6) and is involved in mTOR signaling by coordinating the regulation of nutrient availability with gene expression (1). In accord with its ability to coordinate gene expression with nutrient availability, RMP was most recently shown to be a mitochondrial substrate of S6K1. S6K1-mediated phosphorylation of RMP at Ser371 triggers a series of biochemical events that constitute a negative feedback loop, in part, aimed at restraining S6K1 survival signaling and ensuring that the mitochondrial threshhold for apoptosis corresponds to availability of nutrients and growth factors (7).
Catalog #R2039-21N
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to residues near the C-terminus of human RMP.
SpecificityRecognizes endogenous levels of total human RMP. Species Crossreactivity: Monkey, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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