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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum E B IF

High density lipoproteins (HDLs) play a critical role in cholesterol metabolism. HDL plasma concentrations are inversely correlated with risk for atherosclerosis. SR-BI and SR-BII (previously known as SR-BI.2) are the alternatively spliced products of a single gene. SR-BII differs from SR-BI in that the encoded c-terminal cytoplasmic domain is almost completely different. SR-BII binds HDLs mediating selective uptake of HDL cholesteryl ester; with an approximately 4-fold lower efficiency than SR-BI. Nuclease protection assays show SR-BII to be abundant in mouse tissues expressing SR-BI. SR-BII expression found in liver, adrenal glands, and testes. The role of SR-BII is not completely clear. Research suggests that it may be a functional HDL receptor. SR-BII mRNA results from the alternative splicing of SR-BI precursor transcripts with the SR-BII isoform mediating selective transfer of lipid between HDL and cells. The relative expression and functional activities of these two isoforms create a potential means of regulating selective lipid transfer between HDL and cells (1-6).
Catalog #	S0099-010
Applications	Suitable for use in Western Blot and Immunofluorescence. Other applications not tested
Recommended Dilution	Western Blot: 1:500. A band of ~82kD is seen in tissues that express SR-BII such as liver, tested and adrenal glands.
	Immunofluorescence: 1:250-1:500
	Optimal dilution determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	Not determined
Form	Supplied as a liquid, serum, 0.05% sodium azide, before the addition of glycerol to 40%.
Purity	Serum
Immunogen	Synthetic peptide cooresponding to aa491-506
(CLLEDSLSGQPTSAMA-COOH) of mouse SR-BII with an N-terminal cysteine added for KLH coupling.
Specificity	Specific for SR-BII. Species crossreactivity: Mouse, rat, bovine and hamster.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.