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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Sarcoglycan, delta

Anti -Sarcoglycan, delta

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Delta-sarcoglycan is one of six known sarcoglycans, which are type I (alpha and epsilon) or type II (beta, gamma, delta or zeta) transmembrane glycoproteins. Sarcoglycans associate in different combinations in a tissue-specific manner, to form heterotetrameric, pentameric and hexameric membrane glycoprotein complexes (SGC). SGC interacts laterally with the dystroglycan complex, and both complexes are components of the dystrophin-associated glycoprotein complex (DGC), which forms a link between the cytoskeleton and the extracellular matrix. Mutations in sarcoglycans are associated with recessive autosomally inherited muscular dystrophy.
Catalog #S0160
ApplicationsSuitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1.0ug/ml; Detection limit is ~0.3ng/well
Western Blot: 0.1-0.2ug/ml; Detection limit is ~5ng/lane (non-reducing) and 1ng/lane (reducing)
Immunohistochemistry: 10ug/ml; Detects delta-Sarcoglycan in human fetal heart tissue sections. Sections were fixed with PBS, 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted S0160 overnight at 4°C followed by Rhodamine Red-coupled anti-goat IgG at RT in the dark for 1 hr. Between each step, cells were washed with PBS, 1% BSA.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.2, 5% trehalose. Reconstitute with 500ul 40-50% glycerol, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenPurified, E. coli-derived, recombinant human delta Sarcoglycan isoform 1 (rh delta-Sarcoglycan) extracellular domain.
SpecificityRecognizes human delta-Sarcoglycan extracellular domain.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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