Delta-sarcoglycan is one of six known sarcoglycans, which are type I (alpha and epsilon) or type II (beta, gamma, delta or zeta) transmembrane glycoproteins. Sarcoglycans associate in different combinations in a tissue-specific manner, to form heterotetrameric, pentameric and hexameric membrane glycoprotein complexes (SGC). SGC interacts laterally with the dystroglycan complex, and both complexes are components of the dystrophin-associated glycoprotein complex (DGC), which forms a link between the cytoskeleton and the extracellular matrix. Mutations in sarcoglycans are associated with recessive autosomally inherited muscular dystrophy.
Applications
Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Direct ELISA: 0.5-1.0ug/ml; Detection limit is ~0.3ng/well Western Blot: 0.1-0.2ug/ml; Detection limit is ~5ng/lane (non-reducing) and 1ng/lane (reducing) Immunohistochemistry: 10ug/ml; Detects delta-Sarcoglycan in human fetal heart tissue sections. Sections were fixed with PBS, 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted S0160 overnight at 4°C followed by Rhodamine Red-coupled anti-goat IgG at RT in the dark for 1 hr. Between each step, cells were washed with PBS, 1% BSA. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to human delta Sarcoglycan isoform 1 Met1-Glu232, expressed in E. coli.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 5% trehalose. Reconstitute with 500ul PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human delta-Sarcoglycan extracellular domain.