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S1010-86A Rabbit Anti-SGLT-1 (Sodium Glucose Transporter 1) (Not for Export EU)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Serum
Applications
E
Crossreactivity
Bo Ca Fe Hu Mo Po Rb Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

The kidneys play a major role in the regulation of glucose levels. Kidneys filter ~180g of glucose per day from the blood, and this is mostly reabsorbed back into the blood in the proximal tubules. Typically, glucose is first absorbed within epithelium by a specific transporter protein, sodium glucose co-transporters (SGLT), in the brush-border membrane and then it is transported out of the cell across the basolateral membranes bya facilitated sugar transporter (GLUTs). 3 members of SGLTs (SGLT1-3) have been cloned and characterized from various species. Individual members of this family have identical predicted secondary structures with up to 14 transmembrane domains. SGLT1-3 genes code for protein of ~659-672 residues (75kD). Both N and C termini are predicted to be extracellular. There is ~60-70% homology between SGLT1-3. SGLTs transport a-methyl-D-glucoside (a-MDG), a non metabolized model substrate, in Na-dependent manner. SGLT1 does not discriminate a-MDG, glucose, and galactose. SGLT2/3 do not transport D-galactose efficiently.

Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000-1:5000, ~70-77kD. ELISA: 1:10,000-1:50,000. Coat ELISA plates with control peptide (S1010-86F) at 1ug/ml. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 19aa near the extracellular domain 4 of mouse/rabbit SGLT-1, conjugated to KLH.
Form
Supplied as a liquid in 0.05% sodium azide
Purity
Serum
Specificity
Recognizes mouse SGLT-1. Species Crossreactivity: rabbit, rat, human, porine, feline, canine, reptiles and birds. |Species Sequence Homology: rabbit and sheep; 100%, equine and rat; 94%, porcine; 89%, chicken; 86%, human; 84%. mouse SGLT-4a/4b; 84%, human SAAT/SGLT-3; 78%, human and rat SGLT-2; 63%
References
1. Hediger, MA et al (1987) Nature 330, 1379-1381; 2. Sileverman , M et al (1993) BBA 1153, 43-52. 3. Pajor, AM et al (1992) Am. J. Physiol. 32, R489-R495. 4. Hirayama, EA et al (1992) BBA 1103, 37-44. 5. Hirayama et al (1991) Am. J. Physiol. 261, C296-C304.
USBio References
No references available
Conjugates
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