| SLP 76 is one of several adaptor proteins assisting with TCR-mediated signal transduction, through Grb2 binding, functioning as a substrate for the protein-tyrosine kinase ZAP-70 and thus playing a crucial role in both T cell ontogeny and activation. Other molecules that associate with SLP 76 include phospholipase C (PLC)–gamma, Vav, Nck and fyn binding protein. |
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| Catalog # | S1014-20D |
| SLP 76 was recently identified as a substrate of the T cell receptor stimulated protein tyrosine kinase. This molecule is rapidly phosphorylated on T cell receptor activation. SLP 76 has three identifiable domains; the first containing a number of tyrosines which are the likely sites of phosphorylation, the second a proline-rich domain, and the third an SH2 domain. SLP 76 has been shown to bind to the Grb2 adapter protein in vivo; the association between Grb2 and SLP 76 has been mapped to a 20 amino acid stretch in SLP76's proline-rich region. The SH2 domain of SLP 76 binds to two tyrosine phosphorylated proteins after T cell receptor activation. These molecules are yet to be identified. The SH2 domain of SLP 76 also appears to bind to a serine threonine kinase, perhaps via an indirect association.. Interestingly, overexpression of SLP 76 results in greatly augmented IL-2 production in transiently transfected T cells. Collectively, these data suggest that SLP 76 plays an important role in T cell activation and perhaps is involved in coupling signaling pathways after activation of the T cell antigen receptor. Immunogen | Bacterially expressed GST-SLP 76 fusion protein translated from the cloned human cDNA. |
| Applications | Suitable for use in Western Blot and Immunohistochemistry (frozen). Other applications not tested. |
| Recommended Dilution | Western Blot: 1:500, detects a band of approximately 76kD in T lymphocyte cell lysates. |
| Optimal dilution determined by the researcher. |
| Recommended Secondary Reagents | F(ab')2 rabbit anti mouse IgG:HRP |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Monoclonal |
| Isotype | IgG2b |
| Clone No | 6A462 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | A fusion protein corresponding to amino acids 21.6-434 of human SLP-76. |
| Specificity | Recognizes the SH2 domain-containing leucocyte protein (SLP-76), a 76kD tyrosine phosphoprotein, expressed primarily by T lymphocytes and cells of the myeloid lineage. Species Cross Reactivity: Human, Mouse, Porcine. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
