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You are here:Home » Antibodies » Abs to Adapter Proteins » Anti -SLP 76 (SH2-domain Leukocyte Protein)

Anti -SLP 76 (SH2-domain Leukocyte Protein)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
SLP 76 is one of several adaptor proteins assisting with TCR-mediated signal transduction, through Grb2 binding, functioning as a substrate for the protein-tyrosine kinase ZAP-70 and thus playing a crucial role in both T cell ontogeny and activation. Other molecules that associate with SLP 76 include phospholipase C (PLC)–gamma, Vav, Nck and fyn binding protein.
Catalog #S1014-20D
SLP 76 was recently identified as a substrate of the T cell receptor stimulated protein tyrosine kinase. This molecule is rapidly phosphorylated on T cell receptor activation. SLP 76 has three identifiable domains; the first containing a number of tyrosines which are the likely sites of phosphorylation, the second a proline-rich domain, and the third an SH2 domain. SLP 76 has been shown to bind to the Grb2 adapter protein in vivo; the association between Grb2 and SLP 76 has been mapped to a 20 amino acid stretch in SLP76's proline-rich region. The SH2 domain of SLP 76 binds to two tyrosine phosphorylated proteins after T cell receptor activation. These molecules are yet to be identified. The SH2 domain of SLP 76 also appears to bind to a serine threonine kinase, perhaps via an indirect association.. Interestingly, overexpression of SLP 76 results in greatly augmented IL-2 production in transiently transfected T cells. Collectively, these data suggest that SLP 76 plays an important role in T cell activation and perhaps is involved in coupling signaling pathways after activation of the T cell antigen receptor. Immunogen Bacterially expressed GST-SLP 76 fusion protein translated from the cloned human cDNA.
ApplicationsSuitable for use in Western Blot and Immunohistochemistry (frozen). Other applications not tested.
Recommended DilutionWestern Blot: 1:500, detects a band of approximately 76kD in T lymphocyte cell lysates.
Optimal dilution determined by the researcher.
Recommended Secondary ReagentsF(ab')2 rabbit anti mouse IgG:HRP
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No6A462
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenA fusion protein corresponding to amino acids 21.6-434 of human SLP-76.
SpecificityRecognizes the SH2 domain-containing leucocyte protein (SLP-76), a 76kD tyrosine phosphoprotein, expressed primarily by T lymphocytes and cells of the myeloid lineage. Species Cross Reactivity: Human, Mouse, Porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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