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S1014-53B1 Rabbit Anti-Smad2, phosphorylated (Ser465, Ser467) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
4°C (-20°C Glycerol)

Smad2 (58kD) is a family member of proteins involved in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: (1) the receptor-regulated Smads (activin/TGF-beta receptor-regulated [Smad2 and 3] or BMP receptor regulated [Smad1, 5 and 8]); (2) the common partner (Smad4), that functions via its interaction to the various Smads; and (3) the inhibitory Smads, (Smad6 and Smad7). Smad2 consists of two highly conserved domains, the Nterminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains. The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain, conserved among all the Smads, regulates Smad2 oligomerization and binding to cytoplasmic adaptors and transcription factors. Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGF-beta signaling. Phosphorylation of the two TGF-beta-dependent serines 465 and 467 in the C-terminus of Smad2 provides a recognition site for interaction with Smad4. The phosphorylation of Smad2 on these two serine sites is critical for Smad2 transcriptional activity and TGF--beta signaling.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Positive Control: HepG2 cells + TGF-beta
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer..
Immunogen
Synthetic phosphopeptide derived from a region of human Smad2 that contains serines 465 and 467. The sequence is conserved in mouse and rat.
Form
Supplied as a liquid in Dulbecco’s PBS (without Mg2+ and Ca2+), pH 7.3,1mg/ml BSA (IgG, protease free), 0.09% sodium azide 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes Human Smad2.when phosphorylated at both Ser465 and Ser467. No crossreactivity with Smad3 (80% homologous) Some cross-reactivity may occur in cell systems expressing a high level of Smad3. Species sequence homology: mouse and rat.100%
References
1. Cordenonsi, M., et al. (2003) Links between tumor suppressors. p53 is required for TGF-beta gene responses by cooperating with Smads. Cell 113(3):301-314.||2. Cao, Z., et al. (2003) Levels of phospho-Smad2/3 are sensors of the interplay between effects of TGF-beta and retinoic acid on monocytic and granulocytic differentiation of HL-60 cells. Blood 101(2):498-507.||3. Blanchette, F., et al. (2001) Cross-talk between the p42/p44 MAP kinase and Smad pathways in transforming growth factor beta 1-induced furin gene transactivation. J. Biol. Chem. 276(36):33986- 33994.||4. Nomura, M. and E. Li (1998) Smad2 role in mesoderm formation, left-right patterning and craniofacial development. Nature 393(6687):786-790.||5. Souchelnytskyi, S., et al. (1997) Phosphorylation of Ser465 and Ser467 in the C terminus of Smad2 mediates interaction with Smad4 and is required for transforming growth factor-beta signaling. J. Biol. Chem. 272(44):28107-28115.||6. Abdolla, S., et al. (1997) TbetaRI phosphorylation of Smad2 on Ser465 and Ser467 is required for Smad2-Smad4 complex formation and signaling. J. Biol. Chem. 272(44):27678-27685. ||7. Nakao, A., et al. (1997) Identification of Smad2, a human Mad-related protein in the transforming growth factor beta signaling pathway. J. Biol. Chem. 272(5):2896-2900.
USBio References
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