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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -Smad3, phosphorylated (Ser423/425) (SMAD Family Member 3, DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HSPC193, HsT17436, JV15-2, Mad3, MADH3, MGC60396, Mothers Against Decapentaplegic Homolog 3, Mothers Against DPP Homolog 3)

Anti -Smad3, phosphorylated (Ser423/425) (SMAD Family Member 3, DKFZp586N0721,
DKFZp686J10186, hMAD-3, hSMAD3, HSPC193, HsT17436, JV15-2, Mad3, MADH3, MGC60396, Mothers
Against Decapentaplegic Homolog 3, Mothers Against DPP Homolog 3)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B
Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-beta signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses. Following stimulation by TGF-beta, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-beta Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression.
Catalog #S1014-53P2
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid, 0.05% sodium azide.
PuritySerum.
ImmunogenSynthetic phospho-peptide corresponding to residues surrounding Ser423/425 of human Smad3 (KLH). Cellular Localization: Cytoplasm.
SpecificityRecognizes phosphorylated Smad3 (Ser423 and Ser425). Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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