Anti -Smad3, phosphorylated (Ser423/425) (SMAD Family Member 3, DKFZp586N0721,
DKFZp686J10186, hMAD-3, hSMAD3, HSPC193, HsT17436, JV15-2, Mad3, MADH3, MGC60396, Mothers
Against Decapentaplegic Homolog 3, Mothers Against DPP Homolog 3)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
B |
|
| Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-beta signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses. Following stimulation by TGF-beta, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-beta Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression. | | | Catalog # | S1014-53P2 | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid, 0.05% sodium azide. | | Purity | Serum. | | Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser423/425 of human Smad3 (KLH). Cellular Localization: Cytoplasm. | | Specificity | Recognizes phosphorylated Smad3 (Ser423 and Ser425). Species Crossreactivity | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Smad3, phosphorylated (Ser423/425) (SMAD Family Member 3, DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HSPC193, HsT17436, JV15-2, Mad3, MADH3, MGC60396, Mothers Against Decapentaplegic Homolog 3, Mothers Against DPP Homolog 3) |
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