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You are here:Home » Antibodies » Abs to Sprouty » Anti -Sprouty 1 (hSPRY1, Sprouty Homolog 1 Antagonist of FGF Signaling, Protein Sprouty Homolog 1, Sprouty Homolog 1, SPRY1, Spry-1)

Anti -Sprouty 1 (hSPRY1, Sprouty Homolog 1 Antagonist of FGF Signaling, Protein Sprouty
Homolog 1, Sprouty Homolog 1, SPRY1, Spry-1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
SPRY1 is a 34-38kD member of the sprouty family of proteins. It is widely expressed, being found in multiple embryonic and adult tissues. SPRY1 is considered a negative regulator of cellular signaling. In particular, it appears to both inhibit MAP kinase signaling following RTK activation, and block TCR signaling following antigen activation. It interacts with a number of molecules, including PLC-g 1, LAT, CBL, caveolin-1 and SPRY2. Human SPRY1 is 319aa in length. It contains one CBL-TKB binding site aa51-57 that is phosphorylated at Tyr53, a Ser-rich region aa112-131, and a Cys-rich domain aa181-306 that mediates intracellular translocation. SPRY1 undergoes serine phosphorylation, ubiquitination and palmitoylation, the latter which induces SPRY1 to associate with cell membranes. Over aa1-178, human SPRY1 shares 76% aa identity with mouse SPRY1.
Catalog #S5620-01G
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:16,000
Western Blot: 1-3ug/ml
Immunohistochemistry (Paraffin): 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to CPSRGQGKPS, from SPRY1, at C-terminal (NP_005832.1, NP_955359.1).
SpecificityRecognizes human SPRY1. Species Crossreactivity: Mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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