USBio Logo

S6505-15 Mouse Anti-SREBP-1 (Sterol Regulatory Element Binding Protein-1, SREBP1, ADD1, D630008H06, Sterol Regulatory Element Binding Transcription Factor 1, SREBF1)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
3F335
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hm Hu
Gene #
SREBF1
Shipping Temp
Blue Ice
Storage Temp
-20°C

SREBP-1 and-2 are transcription factors which participate in the control of cholesterol homeostasis. SREBP proteins are attached to the endoplasmic reticulum and nuclear envelope. They are proteolytically cleaved and activated in response to conditions of low cellular sterol. Upon activation of SREBP-1 or -2, an ~480-500aa (N-terminal cleavage fragment of these proteins) enters the nucleus, activating transcription of enzymes and other proteins required for cholesterol synthesis. SCA (SREBP-cleavage activity) and caspase-3 proteases cleave SREBPs. SREBP proteins containing point mutations at caspase-3 cleavage sites (Asp460 in SREBP-1 and Asp468 in SREBP-2) do not become cleaved following induction of apoptosis. This suggests that SREBPs may play some role in apoptotic processes.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1-2ug/ml for 2hrs at RT. Optimal dilution determined by the researcher.
Positive Control
HeLa and IMR5 cells
Cellular Localization
Cytoplasmic and nuclear
MW of Antigen
125kD (precursor) 60-70kD (cleaved)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein encoding N-terminal aa301-407 (the bHLH/leucine zipper domain) of human SREBP-1. Epitope: aa301-407. MW of Antigen: Precursor: 125kD; Cleaved: 60-70kD.|Cellular Localization: Cytoplasmic and nuclear.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from ascites.
Specificity
Recognizes human SREBP-1. Species Crossreactivity: hamster.
References
1. Wang, X., et al., J. Biol. Chem. 270: 15044-18050 (1995). 2. Wang, X., et al., EMBO J. 15: 1012-1020 (1996). 3. Sato, R., et al., J. Biol. Chem. 269: 1726-17273 (1994).
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved