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S7969-08 Rabbit Anti-STAT 1, phosphorylated (Tyr701) (Signal Transducer and Activator of Transcription 1)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Swiss Prot
P42225 (mouse), P42224 (human)
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Ch Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

STAT proteins (Signal Transduction and Activators of Transcription) are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway – a major pathway of the immune system. All cytokines transduce critical signals through this pathway. STAT 1, is activated by a number of different ligands, including IFNalpha, IFNgamma, EGF, PDGF and IL-6. Phosphorylation of tyrosine 701 is required for STAT 1 dissociation from IFNGR1, homodimerization, and nuclear translocation. Tyrosine 701 phosphorylation impairment results in loss of STAT 1 functions.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1:500-1:2000 detects phosphorylated STAT1 in RIPA lysates from CNTF-treated SK-N-MC cells. 20ug of non-treated or CNTF-treated SK-N-MC cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with S7969-08 (1:1000). Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system.
Control
Mouse 3T3-L1 adipocytes stimulated with leukemia inhibitory factor (LIF)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa694-705, C-DDPKRTG[pY]IKTE of mouse STAT1, containing a N-terminal cysteine conjugated to KLH
Form
Supplied as a liquid in PBS, pH 7.6, 5mg/ml BSA, 0.09% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography
Specificity
Recognizes mouse phosphorylated STAT1 at ~92kD. Species Crossreactivity: human, rat and chicken
References
1. Darnell Jr., J.E., et al., Science 264: 1415-1421 (1994). 2. Ihle, J.N., Nature 377: 591-594 (1995). 3. Hosui, A., et al., J. Biol. Chem. 278: 28,562-28,571 (2003).
USBio References
No references available
Conjugates
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