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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 1, phosphorylated (Tyr701) (Signal Transducer and Activator of Transcription 1)

Anti -STAT 1, phosphorylated (Tyr701) (Signal Transducer and Activator of Transcription 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
STAT proteins (Signal Transduction and Activators of Transcription) are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway a major pathway of the immune system. All cytokines transduce critical signals through this pathway. STAT 1, is activated by a number of different ligands, including IFNalpha, IFNgamma, EGF, PDGF and IL-6. Phosphorylation of tyrosine 701 is required for STAT 1 dissociation from IFNGR1, homodimerization, and nuclear translocation. Tyrosine 701 phosphorylation impairment results in loss of STAT 1 functions.
Catalog #S7969-08
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:5001:200 dilution detects phosphorylated STAT1 in RIPA lysates from CNTF-treated SK-N-MC cells. 20ug of non-treated or CNTF-treated SK-N-MC cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with S7969-08. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
ControlMouse 3T3-L1 adipocytes stimulated with leukemia inhibitory factor (LIF)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid in PBS, 5mg/ml BSA, 0.1% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa694-705, C-DDPKRTG[pY]IKTE of mouse STAT1, containing a N-terminal cysteine conjugated to KLH.
SpecificityRecognizes mouse phosphorylated STAT1 at ~92kD. Species Crossreactivity: human, rat and chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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