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S7972-07C Rabbit Anti-STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P42229
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Signal Transducer and Activator of Transcription 5 (STAT5, consists of isoforms a and b) is a ~95kD member of a family of transcription factors that are activated to regulate gene transcription in response to multiple hematopoietic cytokines and growth factors. STAT5 is involved in normal cell cycle, survival and apoptosis and is implicated in leukaemogenesis and angiogenesis, as it has been found to be constitutively active in many leukemic cell lines. STAT5 is activated by phosphorylation of tyrosine 694 in STAT5a (tyrosine 699 in STAT5b), a major Src phosphorylation site that is critical for STAT5 dimerization, translocation and binding to DNA. Phosphorylation of tyrosine 694 is also important in mediating erythropoietin-induced signaling and prolactin-induced development and differentiation of mammary epithelial cells.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000. Optimal dilutions to be determined by the researcher.
Positive Control: 3T3-L1 adipocytes +/- Leukemia Inhibitory Factor (LIF).
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic phosphopeptide derived from the region of human STAT5a that contains tyrosine 694.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.3, 0.05% sodium azide, 50% glycerol. Also available without BSA & Azide. See S7972-07C1.
Purity
Purified by sequential epitope-specific chromatography.
Specificity
Recognizes human STAT5 phosphorylated at Tyr694. Species Sequence Homology: mouse STAT5; human and rat STAT5a: 100%; human STAT5b: 100%; mouse and rat STAT5b: 92%.
References
1. Gewinner, C., et al. (2004) The coactivator of transcription CREB-binding protein interacts preferentially with the glycosylated form of Stat5. J. Biol. Chem. 279(5):3563-3572. 2. Gutierrez-Castellanos, S., et al. (2004) Differences in BCL-X(L) expression and STAT5 phosphorylation in chronic myeloid leukaemia patients. Eur. J. Haematol. 72(4):231-238. 3. Xu, W., et al. (2004) Geldanamycin, a heat shock protein 90-binding agent, disrupts Stat5 activation in IL-2-stimulated cells. J. Cell. Physiol. 198(2):188-196. 4. Rico-Bautista, E., et al. (2004) Downregulation of the growth hormone-induced Janus kinase 2/signal transducer and activator of transcription 5 signaling pathway requires an intact actin cytoskeleton. Exp. Cell. Res. 294(1):269-280. 5. Heath, C. and N.C. Cross (2004) Critical role of STAT5 activation in transformation mediated by ZNF198-FGFR1. J. Biol. Chem. 279(8):6666-6673. 6. Shipley, J.M. and D.J. Waxman (2003) Down-regulation of STAT5b transcriptional activity by ligand-activated peroxisome proliferator-activated receptor (PPAR) alpha and PPARgamma. Mol. Pharmacol. 64(2):355-364. 7. Okutani, Y., et al. (2001) Src directly tyrosine-phosphorylates STAT5 on its activation site and is involved in erythropoietin-induced signaling pathway. Oncogene 20(45):6643-6650.8. Gesbert, F. and J.D. Griffin (2000) Bcr/Abl activates transcription of the Bcl-X gene through STAT5. Blood 96(6):2269-2276.
USBio References
No references available
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