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S8060-02B Rabbit Anti-Superoxide Dismutase, Cu,Zn (Superoxide Dismutase [Cu-Zn], SOD Cu,Zn, SOD1)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Bovine
Swiss Prot
P00441
Isotype
IgG
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Bo Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
EC=1.15.1.1

Superoxide dismutase (SOD) is a 152aa enzyme previously known as haemocuprein or erythrocuprein and belongs to the Cu-Zn superoxide dismutase family. In mammals, it was first described in bovine erythrocytes and is a homodimeric non-covalently bound protein where each monomer has one intrachain disulphide and one free sulfhydryl, and two Cu+2 atoms and two Zn+2 atoms. SOD catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen and thus plays an important role in the defense of cells against the toxic effects of oxygen radicals. It competes with nitric oxide (NO) for superoxide anion, thus promoting the activity of NO. SOD may also play a critical role in suppressing apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice by preventing the conversion of NO to peroxynitrite.

Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 0.025-0.1ug/ml Immunohistochemistry (FFPE): 5ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human, mouse or rat liver lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Superoxide dismutase [Cu-Zn] enzyme from bovine erythrocytes (CAS #9054-89-1)
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes bovine SOD1. Species Crossreactivity: human, mouse and rat
References
1. Beckman, JS. et al. Proc. Natl. Acad. Sci. USA. 87:1620-1624 (1990). 2. Tilly, JL. et al. Endocrinology. 136:242-252 (1995). 3. Keller, JN. et al. J. Neurosci. 18:687-697 (1998).
USBio References
No references available
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