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You are here:Home » Antibodies » Abs to Superoxide Dismutase » Anti -Superoxide Dismutase, Mn (SOD Mn)

Anti -Superoxide Dismutase, Mn (SOD Mn)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B FC
Superoxide dismutase (SOD) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide (1, 2, 3). There are three SOD isoenzymes in mammalian cells. They are: extracellular SOD (EC SOD), copper and zinc-containing SOD (CuZn SOD) and manganese-containing SOD (Mn SOD, SOD-2). The CuZn form contains Cu and Zn ions and exists as a 32kD dimer in the cytosol. Mn SOD is an 80kD tetramer that contains Mn ion and resides in the mitochondrial matrix. Mn SOD is a tumor necrosis factor (TNF)-inducible enzyme that protects cells from TNF-mediated apoptosis (4) via superoxide anion detoxification and the subsequent regulation of apoptosis through cytochrome c release and the modulation of the redox state of the mitochondria (6). Mn SOD has also been shown to be a tumor suppressor in human breast cancer (7). Overexpression of this enzyme protects neurons from NMDA-and nitric oxide-induced neurotoxicity (5).
Applications:
Suitable for use in Western Blot and Flow Cytometry. Other applications not tested.
Catalog #S8060-10B
Recommended DilutionWestern Blot (Colorimetric) (8): 0.5ug/ml
Flow Cytometry: 10ug/ml.
Optimal dilutions to be determined by researcher.
Positive Control:
Rat Brain Tissue Extract
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.0, 15mg/ml BSA, 0.1% sodium azide, 50% glycerol.
PurityPurified by affinity chromatography.
ImmunogenRat Mn SOD8.
SpecificityRecognizes a rat ~25kD protein, corresponding to the apparent molecular mass of rat Mn superoxide dismutase (SOD). Species Crossreactivity: human, mouse, bovine, canine, chicken, Drosophila, guinea pig, hamster, monkey, porcine, rabbit, sheep and Xenopus.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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