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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B FC

Superoxide dismutase (SOD) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide (1, 2, 3). There are three SOD isoenzymes in mammalian cells. They are: extracellular SOD (EC SOD), copper and zinc-containing SOD (CuZn SOD) and manganese-containing SOD (Mn SOD, SOD-2). The CuZn form contains Cu and Zn ions and exists as a 32kD dimer in the cytosol. Mn SOD is an 80kD tetramer that contains Mn ion and resides in the mitochondrial matrix. Mn SOD is a tumor necrosis factor (TNF)-inducible enzyme that protects cells from TNF-mediated apoptosis (4) via superoxide anion detoxification and the subsequent regulation of apoptosis through cytochrome c release and the modulation of the redox state of the mitochondria (6). Mn SOD has also been shown to be a tumor suppressor in human breast cancer (7). Overexpression of this enzyme protects neurons from NMDA-and nitric oxide-induced neurotoxicity (5).
Applications:
Suitable for use in Western Blot and Flow Cytometry. Other applications not tested.
Catalog #	S8060-10B
Recommended Dilution	Western Blot (Colorimetric) (8): 0.5ug/ml
	Flow Cytometry: 10ug/ml.
	Optimal dilutions to be determined by researcher.
	Positive Control:
	Rat Brain Tissue Extract
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.0, 15mg/ml BSA, 0.1% sodium azide, 50% glycerol.
Purity	Purified by affinity chromatography.
Immunogen	Rat Mn SOD8.
Specificity	Recognizes a rat ~25kD protein, corresponding to the apparent molecular mass of rat Mn superoxide dismutase (SOD). Species Crossreactivity: human, mouse, bovine, canine, chicken, Drosophila, guinea pig, hamster, monkey, porcine, rabbit, sheep and Xenopus.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.