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S9200-02 Mouse Anti-Syntaxin (Synaptotagmin-Associated 35kD Protein, Neuron-Specific Antigen HPC-1)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
Q16623
Isotype
IgG1
Clone Number
SP8
Grade
Ascites
Applications
E IHC WB
Crossreactivity
Hm Hu Po Rt
Accession #
NM_004603.2
Shipping Temp
Blue Ice
Storage Temp
-20°C

Syntaxin is membrane integrated Q-SNARE protein participating in exocytosis. Syntaxin is composed of an N-terminal regulatory domain (Habc), a SNARE domain (known as H3), and a single C-terminal transmembrane domain. The SNARE(H3) domain binds to both synaptobrevin and SNAP-25 forming the core SNARE complex.

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 1:500 Western Blot: 1:100-1:10,000 Immunohistochemistry: 1:100-1:200. Formalin fixed (fixed <4 weeks) Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Crude synaptic immunoprecipitate (Human).
Form
Supplied as a liquid in 0.09% sodium azide.
Purity
Ascites
Specificity
Recognizes human Syntaxin (34-36kD). Reacts with syntaxin expressed in bacterial systems. Species Crossreactivity: porcine, hamster and rat.
References
Brain Research (1993) 609:9–20. Neuron (1998) 21:1–20.
USBio References
No references available
Conjugates
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