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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Tapasin (TPN, TPSN, NGS17, NGS-17, TAP-associated Protein, TAPA, TAP-binding Protein, TAPBP)

Anti -Tapasin (TPN, TPSN, NGS17, NGS-17, TAP-associated Protein, TAPA, TAP-binding
Protein, TAPBP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Tapasin is a 48kD glycoprotein which is a member of the immunoglobulin (Ig) superfamily and it is required for efficient peptide binding to Transporter associated with Antigen Processing (TAP). TAP binds peptides in its cytolic part and subsequently translocates the peptides into the lumen of the endoplasmic reticulum (ER) where assembly of MHC class I and peptide takes place. Assembly of MHC class I-beta2-microglobulin (beta2-m) dimers in the ER involves 2 chaperones, calnexin which interacts with free class I heavy (H) chains and calreticulin which binds human class I-beta2 dimers prior to peptide loading. Calreticulin remains associated with at least a subset of class I molecules when they in turn bind to TAP (1). Polymorphic differences in MHC class I H chains can result in quantitative as well as qualitative differences in how they interact with peptide, beta2-m, calnexin, calreticulin, Erp57, TAP and Tapasin, a subunit of the TAP complex which binds to both TAP1 and MHC class I (2,3). Data obtained with Tapasin deletion mutants revealed that binding to TAP is mediated by the C-terminal region and that the N-terminal region is required to stabilize the MHC class I loading complex (4). The Tapasin gene is centromeric of HLA-DP locus between the HSET and HKE1.5 genes and within 500kbp of the transporters associated with antigen processing, TAP1 and TAP2 genes. The localization of these genes within such a short distance of each other on the chromosome implies some regulatory or functional significance (5).
Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Catalog #T0990
Recommended DilutionWestern Blot (ECL): 1:1000
Immunoprecipitation: 1:100
Optimal dilutions to be determined by researcher.
Positive ControlsHeLa Cell Lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot. Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid. in PBS, pH 7.2, 0.09% azide, 50% glycerol
PurityPurified by Protein A affinity chromatography
ImmunogenHuman tapasin synthetic peptide (residues 430-448), with a cysteine (C) residue added.
SpecificityDetects an ~48kD protein, corresponding to the apparent molecular mass of tapasin on SDS-PAGE immunoblots, in samples from human origin.
Species CrossreactivityHuman, mouse and rat. Weakly crossreacts to bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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