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You are here:Home » Antibodies » Abs to Microtubule-associated Proteins » Anti -Tau, 3-repeat isoform RD3 (Tau Protein, Microtubule-associated Protein, MAP) (Azide, BSA & Glycerol Free)

Anti -Tau, 3-repeat isoform RD3 (Tau Protein, Microtubule-associated Protein, MAP) (Azide,
BSA & Glycerol Free)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH
Tau is a heterogeneous group of proteins that range in molecular weight from 50-67kD. They are instumental in the assembly of microtubules and have been associated with some disease states. In normal brain, tau is localized in the axons of the neurons but in some neuropathological lesions, it accumulates within the body of the neuron. Tau protein is a microtubule-associated protein predominantly localized to neuronal axons. Tau promotes tubulin polymerization and the stabilization of microtubules. Hyperphosphorylated forms of Tau are the major components of paired helical filaments (PHF) found in the neurofibrillary tangles which characterize Alzheimer’s disease. Alternate splicing of tau mRNA, glycosylation, and differential phosphorylation contribute to the heterogeneity of tau.
Catalog #T1030-40B1
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Differential Detection of Tauopathies. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000-1:4000 detects Tau (3-repeat isoform RD3) in lysates from rat brain cytosol fraction. Rat brain cytosol fraction was resolved by electrophoresis, transferred to nitrocellulose and probed with T1030-40B1. Proteins were visualized using a IgG (HRP) Gt x Mo and a chemiluminescence detection system.
Immunohistochemistry: Detects Tau (3-repeat isoform RD3) in autoclaved paraffin brain sections (1).
Differential Detection of Tauopathies: See Ref. 2.
Optimal dilutions to be determined by the researcher.
Recommended secondary antibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No4H381
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in PBS. No preservative added. No stabilizing proteins added. Also available with azide. See T1030-40B.
PurityTissue culture supernatant
ImmunogenBovine thyroglobulin conjugated synthetic peptide corresponding to amino acids 209-224, KHQPGGGKVQIVYKPV of human Tau (3-
repeat isoform RD3). Region is flanking junction coded by adjacent exons 9 & 11 in the absence of exon 10.
SpecificityRecognizes human Tau (3-repeat isoform RD3), Mr 45-65kD. Higher MW bands (68-72kD) represent phosphorylated Tau. Species crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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