| Tau is a heterogeneous group of proteins that range in molecular weight from 50-67kD. They are instumental in the assembly of microtubules and have been associated with some disease states. In normal brain, tau is localized in the axons of the neurons but in some neuropathological lesions, it accumulates within the body of the neuron. Tau protein is a microtubule-associated protein predominantly localized to neuronal axons. Tau promotes tubulin polymerization and the stabilization of microtubules. Hyperphosphorylated forms of Tau are the major components of paired helical filaments (PHF) found in the neurofibrillary tangles which characterize Alzheimer’s disease. Alternate splicing of tau mRNA, glycosylation, and differential phosphorylation contribute to the heterogeneity of tau. |
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| Catalog # | T1030-40B1 |
| Applications | Suitable for use in Western Blot, Immunohistochemistry and Differential Detection of Tauopathies. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1:1000-1:4000 detects Tau (3-repeat isoform RD3) in lysates from rat brain cytosol fraction. Rat brain cytosol fraction was resolved by electrophoresis, transferred to nitrocellulose and probed with T1030-40B1. Proteins were visualized using a IgG (HRP) Gt x Mo and a chemiluminescence detection system. |
| Immunohistochemistry: Detects Tau (3-repeat isoform RD3) in autoclaved paraffin brain sections (1). |
| Differential Detection of Tauopathies: See Ref. 2. |
| Optimal dilutions to be determined by the researcher. |
| Recommended secondary antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 4H381 |
| Host | Mouse |
| Source | Human |
| Concentration | Not Determined |
| Form | Supplied as a liquid in PBS. No preservative added. No stabilizing proteins added. Also available with azide. See T1030-40B. |
| Purity | Tissue culture supernatant |
| Immunogen | Bovine thyroglobulin conjugated synthetic peptide corresponding to amino acids 209-224, KHQPGGGKVQIVYKPV of human Tau (3- |
| repeat isoform RD3). Region is flanking junction coded by adjacent exons 9 & 11 in the absence of exon 10. |
| Specificity | Recognizes human Tau (3-repeat isoform RD3), Mr 45-65kD. Higher MW bands (68-72kD) represent phosphorylated Tau. Species crossreactivity |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
