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T1030-40B1 Mouse Anti-Tau, 3-repeat isoform RD3 (Tau Protein, Microtubule-associated Protein, MAP) (Azide, BSA & Glycerol Free)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P10636
Isotype
IgG
Clone Number
4H381 (8E6/C11)
Grade
Supernatant
Applications
IHC WB
Crossreactivity
Hu Rt
Accession #
NM_016835
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free

Microtubule Associated Proteins, or MAPS, bind to the tubulin subunits of microtubule structures and regulate their functional stability. In the cell MAPs bind to monomer and multimerized tubulin. MAP binding to multimerized tubulin further stabilizes the formation of higher order microtubulin structures. MAP binding to microtubule structures is mediated through phosphorylation through Microtubule Affinity Regulated Kinase (MARK). Phosphorylation releases MAPs bound to microtubules, destabilizing the structure, driving it toward disassembly. There are predominately two MAP types, I, II. Type II MAP includes MAP2, MAP4, and tau and are found in nervous tissue. Six tau isoforms exist in brain tissue, and they are distinguished by their number of binding domains. Three isoforms have three binding domains and the other three have four binding domains. The binding domains are located in the carboxy-terminus of the protein and are positively-charged (allowing it to bind to the negatively-charged microtubule). The isoforms with four binding domains are better at stabilizing microtubules than those with three binding domains.

Applications
Suitable for use in Western Blot, Immunohistochemistry and Differential Detection of Tauopathies. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000-1:4000 detects Tau (3-repeat isoform RD3) in lysates from rat brain cytosol fraction. Rat brain cytosol fraction was resolved by electrophoresis, transferred to nitrocellulose and probed with T1030-40B1. Proteins were visualized using a IgG (HRP) Gt x Mo and a chemiluminescence detection system. Immunohistochemistry: Detects Tau (3-repeat isoform RD3) in autoclaved paraffin brain sections (1). Differential Detection of Tauopathies: See Ref. 2. Optimal dilutions to be determined by the researcher.
Recommended secondary antibodies
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa209-224, KHQPGGGKVQIVYKPV of human Tau (3-repeat isoform RD3) conjugated to bovine thyroglobulin. Region is flanking junction coded by adjacent exons 9 & 11 in the absence of exon 10.
Form
Supplied as a liquid in PBS. No preservative added. No stabilizing proteins added. Also available with azide. See T1030-40B.
Purity
Supernatant.
Specificity
Recognizes human Tau (3-repeat isoform RD3), Mr 45-65kD. Higher MW bands (68-72kD) represent phosphorylated Tau. Species crossreactivity: rat. Species sequence homology: mouse and bovine 100%.
References
1. de Silva, R., et al., Neuropathol. Appl. Neurobiol. 29: 288-302 (2003). 2. Togo, T., et al., J. Neuropathol. Exp. Neurol. 61: 547-556 (2002). 3. Hanger, D.P., et al., FEBS Lett. 531: 538-542 (2002).
USBio References
No references available
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