| Thrombospondin is a protein from platelet a-granules. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells. |
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| Catalog # | T5100-06DX |
| Applications | Suitable for use in Electron Microscopy, Flow Cytometry. Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | Immunoprecipitation (Native verified): 2ug/mg protein lysate, Use Protein G. |
| Immunohistochemistry (Formalin/paraffin): 1:25-1:50 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. |
| Western Blot: 1-2ug/ml for 2 hours at RT. |
| Optimal dilution determined by the researcher. |
| Positive Control | Platelets in placenta, megakaryocytes in bone marrow |
| Cellular Localization | Secretory granules, Golgi complex, endoplasmic reticulum, extracellular matrix |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3F353 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See T5100-06D. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Reduced and alkylated puri ed human TSP (fully denatured) from the supernatant of thrombin-activated platelets. MW of Antigen |
| Specificity | Recognizes human Thrombospondin. Shows no crossreactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild crossreaction with TSP 2. Inhibits TSP-collagen interaction. Antibody binding to TSP is unaffected by glycosaminoglycans (e.g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i.e. at low concentration of Ca2+. Species Crossreactivity: bovine, porcine, equine, canine, sheep, mouse and rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
