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You are here:Home » Antibodies » Abs to Thrombospondin » Anti -Thrombospondin (TSP) (BSA & Azide Free)

Anti -Thrombospondin (TSP) (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC IF
Thrombospondin is a protein from platelet a-granules. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.
Catalog #T5100-06DX
ApplicationsSuitable for use in Electron Microscopy, Flow Cytometry. Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunoprecipitation (Native verified): 2ug/mg protein lysate, Use Protein G.
Immunohistochemistry (Formalin/paraffin): 1:25-1:50 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Western Blot: 1-2ug/ml for 2 hours at RT.
Optimal dilution determined by the researcher.
Positive ControlPlatelets in placenta, megakaryocytes in bone marrow
Cellular LocalizationSecretory granules, Golgi complex, endoplasmic reticulum, extracellular matrix
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3F353
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See T5100-06D.
PurityPurified by Protein G affinity chromatography.
ImmunogenReduced and alkylated puri ed human TSP (fully denatured) from the supernatant of thrombin-activated platelets. MW of Antigen
SpecificityRecognizes human Thrombospondin. Shows no crossreactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild crossreaction with TSP 2. Inhibits TSP-collagen interaction. Antibody binding to TSP is unaffected by glycosaminoglycans (e.g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i.e. at low concentration of Ca2+. Species Crossreactivity: bovine, porcine, equine, canine, sheep, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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