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You are here:Home » Antibodies » Abs to Toll Like Receptors (TLR) » Anti -TLR5 (Toll-like Receptor 5, Toll/Interleukin-1 Receptor-like Protein 3, TIL3)

Anti -TLR5 (Toll-like Receptor 5, Toll/Interleukin-1 Receptor-like Protein 3, TIL3)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
TLR5 is an ~100kD type I transmembrane glycoprotein of the Toll-like receptor family. It is expressed on mucosal epithelia in the gastrointestinal tract, airways, and other areas of potential bacterial contact and recognizes bacterial flagellin. Expression is also reported on monocytes, immature dendritic cells, and CD4+ T lymphocytes. The region of human TLR5 used as an immunogen includes the first 2 of 16 leucine-rich repeats and shares 72% aa identity with mouse and rat TLR5. This region is outside of the flagellin binding region. Flagellin engagement induces dimerization and intracellular signaling via MyD88.
Catalog #T8050-45T
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 2.5ug labels 10e6
Immunohistochemistry: 8-25ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No12K188
HostMouse
SourceHuman
Concentration~0.5mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenRecombinant corresponding to aa21-115 from human TLR5 expressed in E.coli.
SpecificityRecognizes human TLR5. Species sequence homology: Recombinant human TLR1, 2, 3, 4, 7, 8, recombinant mouse TLR5, 6, or rhTIRAP (5%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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