Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant E IH

Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
Catalog #	T8065-14
Applications	Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Immunohistochemistry (Formalin/paraffin):1:20 for 20 min at RT. Antigen retrieval of boiling in 10mM citrate buffer, pH 6.0, for 20 min followed by cooling at RT for 20 min is required. Optimal dilutions to be determined by researcher.
Positive Control	Tonsil
Cellular Localization	Nuclear
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	5G228
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in PBS, 0.09% sodium azide before the addition of glycerol to 40%.
Purity	Culture supernatant.
Immunogen	Lysate of the human histiocytic leukemia cell line U937.
Specificity	Recognizes Topoisomerase II alpha. Species Crossreactivity: Human.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.