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You are here:Home » Antibodies » Abs to Topoisomerases » Anti -Topoisomerase II (DNA) alpha

Anti -Topoisomerase II (DNA) alpha

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant E IH
Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
Catalog #T8065-14
ApplicationsSuitable for use in ELISA and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Formalin/paraffin):1:20 for 20 min at RT. Antigen retrieval of boiling in 10mM citrate buffer, pH 6.0, for 20 min followed by cooling at RT for 20 min is required. Optimal dilutions to be determined by researcher.
Positive Control Tonsil
Cellular Localization Nuclear
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No5G228
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, 0.09% sodium azide before the addition of glycerol to 40%.
PurityCulture supernatant.
ImmunogenLysate of the human histiocytic leukemia cell line U937.
SpecificityRecognizes Topoisomerase II alpha. Species Crossreactivity: Human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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