TORC1, also known as CREB-regulated transcription coactivator 1 (crtc1), is a 67kD member of the TORC family. Mouse TORC1 is 630aa in length. It contains multiple potential sites for phosphorylation and a serine-rich region from aa295-365. Mouse TORC1 is 97% and 90% aa identical to rat and human TORC1, respectively. TORC1 is highly expressed in specific regions of the brain including the cortex, hippocampus, and striatum. It functions as a transcription coactivator for CREB1, which activates transcription through both consensus and variant cAMP response element (CRE) sites.
Applications
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: 25ug/ml Optimal dilutions to be determined by the researcher.
Hybridoma
Mouse myeloma cells with B cells obtained from a rat immunized with purified, E. coli-derived, recombinant mouse TORC1 (aa384-471).
Storage and Stability
Lyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
E. coli-derived, recombinant mouse TORC1 (aa384-471; Accession # Q68ED7).
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse TORC1. Species sequence homology: rat (97%), human (90%).