This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein interacts with the hyperphosphorylated C-terminal domain of RNA polymerase II via multiple FF domains, and with the pre-mRNA splicing factor SF1 via a WW domain.
Applications
Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence and Western Blot. Other applications not tested.
Recommended Dilutions
Immunofluorescence (IC): 2-100ug/ml. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. Western Blot: 0.2-2ug/ml Flow Cytometry: 0.5-2ug/ml. HeLa cells with 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant corresponding to a partial sequence of human Transcription Elongation Regulator 1.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant
Specificity
Recognizes human Transcription Elongation Regulator 1. Species Crossreactivity: mouse and rat
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