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You are here:Home » Antibodies » Abs to Transcription Factors, TRIM (Tripartite Motif) » Anti -TRIM5d (Tripartite Motif Protein 5d)

Anti -TRIM5d (Tripartite Motif Protein 5d)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region (1). TRIM5 has five alternately spliced isoforms, the longest of which is the a variant which also contains a carboxy-terminal B30.2 (SPRY) domain (1). Expression of TRIM5a variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV- 1 (2,3), albeit at differing efficiencies. The TRIM5d isoform has been shown to colocalize with the topoisomerase Iinteracting proteins BTBD1 and BTBD2 in punctate or elongated cytoplasmic bodies in several mouse and human cells where it appears to serve as a scaffold for the assembly of endogenous BTBD proteins. TRIM5 d also exhibits autoubiquitination activity in a RING finger- and UbcH5Bdependent manner (4).
Catalog #T8465-20
ApplicationsCan be used for detection of TRIM5d by Western Blot at 1-2ug/ml. Mouse brain cell lysate can be used as a positive control and a band at approximately 25kD can be detected.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to 13 amino acids near the carboxy-terminus of human TRIM5d (Genbank accession No. NP_149084)
SpecificityRecognizes human TRIM5d. Species Crossreactivity: Mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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