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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum B IH

The Trk family of tyrosine protein kinase receptors, is responsible for mediating the trophic effects of the NGF family of neurotrophins.
Catalog #	T8663-20A
Applications	Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended Dilution	Immunohistochemistry (tissue from rat spinal cord): 1:2000-1:3000
	Western Blot (rat membrane preparations): 1:1000-1:1500
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	Not determined
Form	Supplied as a lyophilized powder. No preservative added. Reconstitute with 50ul of sterile dH2O.
Purity	Serum
Immunogen	Synthetic peptide corresponding to aa54-67 (AFPRLEPNSIDPENC) which is a portion of the extracellular domain from rat trkB.
Specificity	Recognizes rat TrkB. The sequence used for the immunogen was chosen to avoid crossreactivity with trkA or trkC. Species Crossreactivity: human
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.