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T8682 Trypsin Inhibitor, Ovomucoid (Egg White) CAS: 9035-81-8

Specifications
References
CAS Number
9035-81-8
Grade
Molecular Biology Grade
MDL Number
MFCD00082102
Molecular Weight
28
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
4°C

Ovomucoids are the glycoprotein protease-inhibitors of avian egg white. There are several protease inhibitors in egg white. One acts upon ficin and papain; another, ovoinhibitor is a significant contaminant of crude ovomucoid preparations and acts upon bovine trypsin and chymotrypsin as well as porcine elastase and fungal proteinase.

Unit Definition
1mg inhibits at least 1.2mg trypsin with at least 180U/mg. (1 unit of Trypsin hydrolyzes 1micromole of TAME/min at 25ºC, pH 8.2, in the presence of 0.1M calcium).
Activity
mg Trypsin inhibited by 1mg of Trypsin Inhibitor
Protein
As Reported
Form
Supplied as a dialyzed, lyophilized powder.
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at 4°C. Reconstitute with sterile ddH2O.
References
1. Kuszak, A.J. et al., (2009) J Biol Chem. 284:26732-26741. 2. Tezel, G. and Wax, M.B. (2000) J. Neuroscience 20:8693-8700. 3. Yang, J. et al., (2000) Curr Eye Res. 21:981-985. Lineweaver, H. and Murray, C. W., J. Biol. Chem., 171:565 (1947). Beeley, J.: Ovomucoid Heterogeneity Examined by Flat Bed Isoelectric Focusing, Biochim. Biophys. Acta, 230, 595 (1971b). Beeley, J.: Cyanogen Bromide Cleavage of Ovomucoid, Biochem. J., 128, 119P (1972). Beeley, J., and Jevons, F.: Heterogeneity of Ovomucoid, Biochim. Biophys. Acta, 101, 133 (1965). Beeley, J., and McCairns, E.: Selective Isolation of Ovomucoid Using an Insolubilized Trypsin Derivative, Biochim. Biophys. Acta, 271, 193 (1972). Bier, M., Terminiello, L., Kuke, J., Gibb, R., and Nord, F.: Investigations on Proteins and Polymers. X. Composition and Fractionation of Ovomucoid, Arch. Biochem. Biophys., 47, 465 (1953). Bode, W., Epp, O., Huber, R., Laskowski, M., and Ardelt, W.: The Crystal and Molecular Structure of the Third Domain of Silver Pheasant Ovomucoid, Eur. J. Biochem., 147, 387 (1985). Davis, J., Mapes, C., and Donovan, J.: Batch Purification of Ovomucoid and Characterization of the Purified Product, Biochem., 10, 39 (1971). Donovan, J.: A Spectrophotometric and Spectrofluorometric Study of Intramolecular Interactions of Phenolic Groups in Ovomucoid, Biochem., 6, 3918 (1967).|Donovan, J., and Beardslee, R.: Heat Stabilization Produced by Protein-Protein Association, J. Biol. Chem., 250, 1966 (1975). Empie, M., and Laskowski, M.: Thermodynamics and Kinetics of Single Residue Replacements in Avian Ovomucoid Third Domains: Effect of Inhibitor Interactions with Serine Proteinases, Biochem., 21, 2274 (1982).|Feeney, R., Osuga, D., and Maeda, H.: Heterogeneity of Avian Ovomucoids, Arch. Biochem. Biophys., 119, 124 (1967).|Feeney, R., Stevens, F., and Osuga, D.: The Specificities of Chicken Ovomucoid and Ovoinhibitor, J. Biol. Chem., 238, 1415 (1963). Feinstein, G., and Gertler, A.: The Effect of Limited Proteolysis of Chicken Ovoinhibitor by Bovine Chymotrypsin on the Inhibitory Activities Against Trypsin, Chymotrypsin and Elastase, Eur. J.|Biochem., 31, 25 (1972). Fossum, K., and Whitaker, J.: Ficin and Papain Inhibitor from Chicken Egg White, Arch. Biochem. Biophys., 125, 367 (1968).||Gertler, A., and Feinstein, G.: Inhibition of Porcine Elastase by Turkey Ovomucoid and Chicken Ovoinhibitor, Eur. J. Biochem., 20, 547 (1971).||Kato, I., Schrode, J., Kohr, W., and Laskowski, M.: Chicken Ovomucoid: Determination of Its Amino Acid Sequence, Determination of the Trypsin Reactive Site, and Preparation of all Three of Its|Domains, Biochem., 26, 193 (1987).||Laskowski, M., Kato, I., Ardelt, W., Cook, J., Denton, A., Empie, M., Kohr, W., Park, S., Parks, K., Schatzley, B., Schoenberger, O., Tashiro, M., Vichot, G., Whatley, H., Wieczorek, A., and|Wieczorek, M.: Ovomucoid Third Domains from 100 Avian Species: Isolation, Sequences, and Hypervariability of Enzyme-Inhibitor Contact Residues, Biochem., 26, 202 (1987).||Lineweaver, H., and Murray, C.: Identification of the Trypsin Inhibitor of Egg White with Ovomucoid, J. Biol. Chem., 171, 565 (1947).||Liu, W., Feinstein, G., Osuga, D., Haynes, R., and Feeney, R.: Modification of Arginines in Trypsin Inhibitors by 1,2-Cyclohexadione, Biochem., 7, 2886 (1968).||Liu, W., Means, G., and Feeney, R.: The Inhibitory Properties of Avian Ovoinhibitors Against Proteolytic Enzymes, Biochim. Biophys. Acta, 229, 176 (1971).||Matsuda, T., Watanabe, K., and Nakamura, R.: Immunochemical Studies on Thermal Denaturation of Ovomucoid, Biochim. Biophys. Acta, 707, 121 (1982).||Matsushima, K.: An Undescribed Trypsin Inhibitor in Egg White, Science, 127, 1178 (1958).||Melamed, M.: Electrophoretic Properties of Ovomucoid, Biochem. J., 103, 895 (1967).||Murthy, G., Gilardeau, C., and Chetien, M.: Partial Sequence of Trypsin Inhibitor from Ovomucoid, Can. J. Biochem., 51, 1548 (1973).||Nagase, H., and Harris, E.: Ovostatin: A Novel Proteinase Inhibitor from Chicken Egg White. II. Mechanism of Inhibition Studied with Collagenase and Thermolysin, J. Biol. Chem., 258, 7490|(1983).||Nagase, H., Harris, E., Woessner, J., and Brew, K.: Ovostatin: A Novel Proteinase Inhibitor from Chicken Egg White. I. Purification, Physicochemical Properties, and Tissue Distribution of|Ovostatin, J. Biol. Chem., 258, 7481 (1983).||Ray, A., Guha, M., and Sinha, N.: Purification and Characterization of an Acidic Trypsin/Subtilisin Inhibitor from Tortoise Egg White, Biochim. Biophys. Acta, 716, 126 (1982).||Rhodes, M., Bennett, N., and Feeney, R.: The Trypsin and Chymotrypsin Inhibitors from Avian Egg Whites, J. Biol. Chem., 235, 1686 (1960).||Tomimatsu, Y., Clary, J., and Barlulovich, J.: Physical Characterization of Ovoinhibitor, a Trypsin and Chymotrypsin Inhibitor from Chicken Egg White, Arch. Biochem. Biophys., 115, 536 (1966).||Travis, J.: Human Pancreatic Enzymes: Interaction of Human Trypsin with Chicken Ovomucoid, Biochem. Biophys. Res. Comm., 44, 793 (1971).||Waheed, A., and Salahuddin, A.: Isolation and Characterization of a Variant of Ovomucoid, Biochem. J., 147, 139 (1975a).||Waheed, A., and Salahuddin, A.: Hydrogen-Ion Titration Curve of Ovomucoid, Biochem. Biophys. Acta, 379, 147 (1975b).||Watanabe,K., Matsuda, T., and Sato, Y.: The Secondary Structure of Ovomucoid and Its Domain as Studied by Circular Dichroism, Biochim. Biophys. Acta, 667, 242 (1981).||Wieczorek, M., and Laskowski, M.: Formation of Covalent Hybrids from Amino-Terminal and Carboxy-Terminal Fragments of Two Ovomucoid Third Domains, Biochem., 22, 2630 (1983).||Zahnley, J.: Evidence That the Two Binding Sites for Trypsin on Chicken Ovoinhibitor are not Equivalent. Dissociation of the Complexes with Porcine Trypsin, J. Biol. Chem., 249, 4282 (1974).||Zahnley, J.: Independent Heat Stabilization of Proteases Associated with Multiheaded Inhibitors: Trypsin Bound at Nonequivalent Sites on Chicken Ovoinhibitor, J. Biol. Chem., 254, 9721 (1979).
USBio References
US Biological application references: 1. Kuszak, A.J. et al., (2009) J Biol Chem. 284:26732-26741. 2. Tezel, G. and Wax, M.B. (2000) J. Neuroscience 20:8693-8700. 3. Yang, J. et al., (2000) Curr Eye Res. 21:981-985.
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