Tubulin

Clone Type

Monoclonal

Host

Mouse

Source

Chicken

Isotype

IgM

Clone Number

5F131

Grade

Supernatant

Applications

E IF IHC WB

Crossreactivity

Ch Hu

Shipping Temp

Blue Ice

Storage Temp

-20°C

Tubulin is found in the microtubules which represent the main structural components of centriols, the mitotic spindle, cilia and flagella.

Applications

Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution

Immunohistochemistry (Frozen): 1:5-1:10. Optimal dilutions to be determined by the researcher.

Positive Control

Cultured fibroblasts (chicken)

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Isolated from chicken muscle cells.

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Purity

Supernatant

Specificity

Recognizes chicken tubulin microtubules in muscle and non- muscle cells. Species Crossreactivity: human

References

Bläckström B and Collins VP: Cytoskeletal changes in axons of rats exposed to 2,5-hexamediol, demonstrated using monoclonal antibodies. Neurotoxicology Vol. 8, 1:85-96 (1987)