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You are here:Home » Antibodies » Abs to Tubulin » Anti -Tubulin, alpha, NT (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

Anti -Tubulin, alpha, NT (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1,
TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IC
The alpha-tubulin (relative molecular weight about 50kD) is globular protein that exists in
cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In
different species it is coded by multiple tubulin genes that form tubulin classes (in human 6
genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes
are expressed ubiquitously, while some have more restricted tissue expression.
Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes
and their posttranslational modifications are responsible for multiple tubulin charge variants -
tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural
domain.The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and
have an outer diameter 25nm. Microtubules have their intrinsic polarity; highly dynamic plus
ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains.
Catalog #T9154-01J
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-2mg/ml under reducing conditions. Prepare samples in Laemmli reducing buffer.
Immunocytochemistry: Use fixed and permeabilized cells.
Optimal dilutions to be determined by the researcher.
WB Positive ControlHPB-ALL peripheral blood leukemia cell lysate (incubation 60 min)
Porcine brain lysate (incubation 90 min)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone NoTU-01
HostMouse
SourcePorcine
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
PurityPurified from ascites by precipitation methods.
ImmunogenFraction of tubulin purified from pig brain
SpecificityRecognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha tubulin.
Species CrossreactivityAll (recognized epitope conserved within all species).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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